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  1. Link

    pdgfr-beta-antibody-apb5-ab91066.pdf

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Cardiovascular Angiogenesis Growth Factors Other
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Anti-PDGFR beta antibody [APB5] (ab91066)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (9)

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Abpromise

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Flow Cytometry - Anti-PDGFR beta antibody [APB5] (ab91066)

    Key features and details

    • Rat monoclonal [APB5] to PDGFR beta
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Recombinant Human PDGFR beta protein (ab185609)
    Secondary
    Product image
    Goat Anti-Rat IgG H&L (HRP) (ab97057)

    View more associated products

    Overview

    • Product name

      Anti-PDGFR beta antibody [APB5]
      See all PDGFR beta primary antibodies
    • Description

      Rat monoclonal [APB5] to PDGFR beta
    • Host species

      Rat
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Fusion protein corresponding to Mouse PDGFR beta.

    • Positive control

      • NIH3T3 cells
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      ab91066 is affinity purified.
    • Clonality

      Monoclonal
    • Clone number

      APB5
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Growth Factors
      • Other
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases
      • Signal Transduction
      • Growth Factors/Hormones
      • PDGF
      • Cancer
      • Growth factors
      • PDGF
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Growth factor receptors
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators

    Associated products

    • Alternative Versions

      • Anti-PDGFR beta antibody [APB5] - Low endotoxin, Azide free (ab171231)
      • APC Anti-PDGFR beta antibody [APB5] (ab93532)
      • Biotin Anti-PDGFR beta antibody [APB5] (ab93533)
      • PE Anti-PDGFR beta antibody [APB5] (ab93534)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
    • Recombinant Protein

      • Recombinant Human PDGFR beta protein (ab185609)

    Applications

    Our Abpromise guarantee covers the use of ab91066 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
  • Application notes
    Flow Cyt: Use 1 µg for 106 cells in 100 µl total staining volume.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 124 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
    • Involvement in disease

      Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
      Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
      Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
      Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
      Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P09619 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5159 Human
      • Omim: 173410 Human
      • SwissProt: P09619 Human
      • Unigene: 509067 Human
      • Alternative names

        • Beta platelet derived growth factor receptor antibody
        • Beta-type platelet-derived growth factor receptor antibody
        • CD 140B antibody
        • CD140 antigen-like family member B antibody
        • CD140b antibody
        • CD140b antigen antibody
        • IBGC4 antibody
        • IMF1 antibody
        • JTK12 antibody
        • OTTHUMP00000160528 antibody
        • PDGF R beta antibody
        • PDGF Receptor beta antibody
        • PDGF-R-beta antibody
        • PDGFR 1 antibody
        • PDGFR antibody
        • PDGFR beta antibody
        • PDGFR1 antibody
        • PDGFRB antibody
        • PGFRB_HUMAN antibody
        • Platelet derived growth factor receptor 1 antibody
        • Platelet derived growth factor receptor beta antibody
        • Platelet derived growth factor receptor beta polypeptide antibody
        see all

      Images

      • Flow Cytometry - Anti-PDGFR beta antibody [APB5] (ab91066)
        Flow Cytometry - Anti-PDGFR beta antibody [APB5] (ab91066)
        Staining of NIH3T3 cells with 0.5 µg control antibody (open histogram) or 0.5 µg ab91066 (colored histogram), followed by biotin-conjugated anti-Rat IgG and Streptavidin PE.
        Total viable cells were used for analysis.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (9)

      Publishing research using ab91066? Please let us know so that we can cite the reference in this datasheet.

      ab91066 has been referenced in 9 publications.

      • Betlazar C  et al. Cellular Sources and Regional Variations in the Expression of the Neuroinflammatory Marker Translocator Protein (TSPO) in the Normal Brain. Int J Mol Sci 19:N/A (2018). PubMed: 30208620
      • McLane LE  et al. Loss of Tuberous Sclerosis Complex1 in Adult Oligodendrocyte Progenitor Cells Enhances Axon Remyelination and Increases Myelin Thickness after a Focal Demyelination. J Neurosci 37:7534-7546 (2017). PubMed: 28694334
      • Shawber CJ  et al. Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus. Vasc Cell 7:9 (2015). PubMed: 26629328
      • Anastasia A  et al. Trkb signaling in pericytes is required for cardiac microvessel stabilization. PLoS One 9:e87406 (2014). IHC-Fr ; Mouse . PubMed: 24498100
      • Zhang Y  et al. An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. J Neurosci 34:11929-47 (2014). PubMed: 25186741
      • Qin D  et al. Early vessel destabilization mediated by Angiopoietin-2 and subsequent vessel maturation via Angiopoietin-1 induce functional neovasculature after ischemia. PLoS One 8:e61831 (2013). Flow Cyt ; Mouse . PubMed: 23613948
      • Rondahl V  et al. Lrig2-deficient mice are protected against PDGFB-induced glioma. PLoS One 8:e73635 (2013). PubMed: 24023893
      • Parsons SP  et al. Maxi-channels recorded in situ from ICC and pericytes associated with the mouse myenteric plexus. Am J Physiol Cell Physiol 302:C1055-69 (2012). IHC-Fr ; Mouse . PubMed: 22159087
      • Sano H  et al. Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice. Circulation 103:2955-60 (2001). PubMed: 11413086

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      IHC - Wholemount abreview for Anti-PDGFR beta antibody [APB5]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      IHC - Wholemount
      Sample
      Mouse Tissue (cochlea)
      Specification
      cochlea
      Read More

      Dr. Xiaorui Shi

      Verified customer

      Submitted Jul 04 2019

      Immunohistochemistry (Frozen sections) abreview for Anti-PDGFR beta antibody [APB5]

      Poor
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Prostate)
      Permeabilization
      No
      Specification
      Prostate
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 29 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR beta antibody [APB5]

      Poor
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Prostate)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
      Specification
      Prostate
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 29 2017

      Immunocytochemistry/ Immunofluorescence abreview for Anti-PDGFR beta antibody [APB5]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Permeabilization
      Yes - 0.5% Triton X100 in PBS
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Apr 04 2014

      Question

      ich habe einen Antikörper von Ihnen erhalten und es steht im Datenblatt,
      dass er mit mouse reagiert.
      Haben sie Erfahrung, ob er auch in human funktioniert? Das wäre sehr
      wichtig für mich.
      Es handelt sich um folgenden Antikörper:
      Anti-PDGF Receptor beta antibody (APB5) (ab91066)

      Read More

      Abcam community

      Verified customer

      Asked on Apr 12 2012

      Answer

      Vielen Dank für Ihre Anfrage.

      Unseres Wissens nach wurde der Antikörper bisher nicht in dieser Spezies getestet. Wir geben alle bekannten Kreuzreaktionen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Ich habe einen Proteinsequenzvergleich zwischen dem mause und humanen Protein durchgeführt, und diese sind zu 100% identisch. Daher ist es sehr wahrscheinlich, dass der Antikörper kreuzreagieren wird. Bitte beachten Sie aber, dass das Immunogen an humanes IgG1 gekoppelt wurde, daher kann es hier zu Kreuzreaktionen kommen. Falls Sie eine IHC durchführen möchten, oder Ihr WB mit einem Gewebelysat durchgeführt wird, möchte ich Ihnen dringend zu einer Negativ- Kontrolle raten.


      Warum teilen Sie uns nicht Ihre Ergebnisse mittels eines „Abreview“ mit? Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken. Wir möchten unsere Kunden immer ermutigen, uns Ihre positiven und auch negativen Ergebnisse mitzuteilen. Wir machen diese Informationen für alle Kunden zugänglich und belohnen „Abreviews“ mit Punkten, die gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden können.

      Unter der folgenden URL können Sie mehr über unser Abreview-System zu erfahren:

      https://www.abcam.com/abreviews



      Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

      Read More

      Abcam Scientific Support

      Answered on Apr 12 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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