Anti-PDGFR beta antibody [APB5] (ab91066)
![Flow Cytometry - Anti-PDGFR beta antibody [APB5] (ab91066)](https://www.abcam.com/ps/products/91/ab91066/Images/ab91066-106667-anti-pdgfr-beta-antibody-apb5-flow-cytometry.jpg "Flow Cytometry - Anti-PDGFR beta antibody [APB5] (ab91066)")
Key features and details
- Rat monoclonal [APB5] to PDGFR beta
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PDGFR beta antibody [APB5]
See all PDGFR beta primary antibodies -
Description
Rat monoclonal [APB5] to PDGFR beta -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Mouse PDGFR beta.
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Positive control
- NIH3T3 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab91066 is affinity purified. -
Clonality
Monoclonal -
Clone number
APB5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab91066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 124 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5159 Human
- Omim: 173410 Human
- SwissProt: P09619 Human
- Unigene: 509067 Human
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Alternative names
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
see all
Images
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Flow Cytometry - Anti-PDGFR beta antibody [APB5] (ab91066)Staining of NIH3T3 cells with 0.5 µg control antibody (open histogram) or 0.5 µg ab91066 (colored histogram), followed by biotin-conjugated anti-Rat IgG and Streptavidin PE.
Total viable cells were used for analysis.
Protocols
References (9)
ab91066 has been referenced in 9 publications.
- Betlazar C et al. Cellular Sources and Regional Variations in the Expression of the Neuroinflammatory Marker Translocator Protein (TSPO) in the Normal Brain. Int J Mol Sci 19:N/A (2018). PubMed: 30208620
- McLane LE et al. Loss of Tuberous Sclerosis Complex1 in Adult Oligodendrocyte Progenitor Cells Enhances Axon Remyelination and Increases Myelin Thickness after a Focal Demyelination. J Neurosci 37:7534-7546 (2017). PubMed: 28694334
- Shawber CJ et al. Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus. Vasc Cell 7:9 (2015). PubMed: 26629328
- Anastasia A et al. Trkb signaling in pericytes is required for cardiac microvessel stabilization. PLoS One 9:e87406 (2014). IHC-Fr ; Mouse . PubMed: 24498100
- Zhang Y et al. An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. J Neurosci 34:11929-47 (2014). PubMed: 25186741
- Qin D et al. Early vessel destabilization mediated by Angiopoietin-2 and subsequent vessel maturation via Angiopoietin-1 induce functional neovasculature after ischemia. PLoS One 8:e61831 (2013). Flow Cyt ; Mouse . PubMed: 23613948
- Rondahl V et al. Lrig2-deficient mice are protected against PDGFB-induced glioma. PLoS One 8:e73635 (2013). PubMed: 24023893
- Parsons SP et al. Maxi-channels recorded in situ from ICC and pericytes associated with the mouse myenteric plexus. Am J Physiol Cell Physiol 302:C1055-69 (2012). IHC-Fr ; Mouse . PubMed: 22159087
- Sano H et al. Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice. Circulation 103:2955-60 (2001). PubMed: 11413086
