Key features and details
- Mouse monoclonal [OTI1D1] to PDGFR beta
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PDGFR beta antibody [OTI1D1]
See all PDGFR beta primary antibodies
DescriptionMouse monoclonal [OTI1D1] to PDGFR beta
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PDGFR beta. Protein produced in HEK293T cells.
MRLPGAMPALALKGELLLLSLLLLLEPQISQGLVVTPPGPELVLNVSSTF VLTCSGSAPV VWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEY FCTHNDSRGLETDERKRLYIFV PDPTVGFLPNDAEELFIFLTEITEIT IPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFS GIFEDRSYICKTTI GDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGN EV VNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCN VTESVNDH QDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVF EAYPPPTVLWFKDNRTLGDS SAGEIALSTRNVSETRYVSELTLVRVKV AEAGHYTMRAFHEDAEVQLSFQLQINVPVRVL ELSESHPDSGEQTVRC RGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNV TYWE EEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFKVVV ISAILA LVVLTIISLIILIMLWQKKPRYEIRWKVIESVSSDGHEYIYV DPMQLPYDSTWELPRDQL VLGRTLGSGAFGQVVEATAHGLSHSQATMK VAVKMLKSTARSSEKQALMSELKIMSHLGP HLNVVNLLGACTKGGPIY IITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNAL PVGLPL PSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYD NYVP SAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVH RDLAARNVLICEGKLV KICDFGLARDIMRDSNYISKGSTFLPLKWMAP ESIFNSLYTTLSDVWSFGILLWEIFTLG GTPYPELPMNEQFYNAIKRG YRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLER LLGEGYKK KYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGD ND YIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEP QDEPEPEPQLELQV EPEPELEQLPDSGCPAPRAEAEDSFL
Database link: NP_002600
- ICC/IF: HeLa cells.
The clone number has been updated from 1D1 to OTI1D1, both clone numbers name the same clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography.
Our Abpromise guarantee covers the use of ab156762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 123 kDa.|
FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
- Information by UniProt
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
Immunofluorescent analysis of HeLa cells labeling PDGFR beta with ab156762 at 1/100.
All lanes : Anti-PDGFR beta antibody [OTI1D1] (ab156762) at 1/1000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PDGF Receptor beta
Lysates/proteins at 5 µg per lane.
Predicted band size: 123 kDa
ab156762 has not yet been referenced specifically in any publications.