Recombinant
RabMAb

Recombinant Anti-PDGFR beta (phospho Y740) antibody [EP2135Y] (ab81301)

Overview

  • Product name

    Anti-PDGFR beta (phospho Y740) antibody [EP2135Y]
    See all PDGFR beta primary antibodies
  • Description

    Rabbit monoclonal [EP2135Y] to PDGFR beta (phospho Y740)
  • Host species

    Rabbit
  • Specificity

    This antibody detects PDGFR beta phosphorylated at Tyrosine 740.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PDGFR beta (phospho Y740). The exact sequence is proprietary.

  • Positive control

    • NIH 3T3 cell lysates.
  • General notes

    Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab81301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 190 kDa (predicted molecular weight: 124 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
    • Involvement in disease

      Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
      Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
      Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
      Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
      Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Beta platelet derived growth factor receptor antibody
      • Beta-type platelet-derived growth factor receptor antibody
      • CD 140B antibody
      • CD140 antigen-like family member B antibody
      • CD140b antibody
      • CD140b antigen antibody
      • IBGC4 antibody
      • IMF1 antibody
      • JTK12 antibody
      • OTTHUMP00000160528 antibody
      • PDGF R beta antibody
      • PDGF Receptor beta antibody
      • PDGF-R-beta antibody
      • PDGFR 1 antibody
      • PDGFR antibody
      • PDGFR beta antibody
      • PDGFR1 antibody
      • PDGFRB antibody
      • PGFRB_HUMAN antibody
      • Platelet derived growth factor receptor 1 antibody
      • Platelet derived growth factor receptor beta antibody
      • Platelet derived growth factor receptor beta polypeptide antibody
      see all

    Images

    • All lanes : Anti-PDGFR beta (phospho Y740) antibody [EP2135Y] (ab81301) at 1/2000 dilution

      Lane 1 : NIH 3T3 un-treated
      Lane 2 : NIH 3T3 treated with PDGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 124 kDa
      Observed band size: 190 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Arango-Lievano M  et al. Topographic Reorganization of Cerebrovascular Mural Cells under Seizure Conditions. Cell Rep 23:1045-1059 (2018). WB, IHC (PFA fixed) ; Mouse . Read more (PubMed: 29694884) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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