Overview

  • Product name
    Anti-PDGFR beta (phospho Y751) antibody
    See all PDGFR beta primary antibodies
  • Description
    Rabbit polyclonal to PDGFR beta (phospho Y751)
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of PGDFR-ß only when phosphorylated at tyrosine 751.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PDGFR beta aa 749-753.
    Sequence:

    VDYVP

  • Positive control
    • WB:A431 cells treated with 0.05ng of PDGF IHC-P: Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab51046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB Use at an assay dependent concentration. Predicted molecular weight: 123 kDa.

Target

  • Function
    Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • Involvement in disease
    Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF Receptor beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

Images

  • All lanes : Anti-PDGFR beta (phospho Y751) antibody (ab51046)

    Lane 1 : A431 cells treated with 0.05ng of PDGF
    Lane 2 : A431 cells untreated treated

    Predicted band size: 123 kDa
    Observed band size: 130-170 kDa
    why is the actual band size different from the predicted?

  • Human brain tissue labelled with ab51046 at 1/50 dilution

References

ab51046 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your message.

I would like to reassure you that although these antibodies are recommended to be stored at -20oC, I would not expect being at 4oC for a few days to significantly affect their performance. However, I would recommend to aliquot and freeze them as soon as possible in order to ensure they remain stable and so we can continue to cover them with our guarantee.

I hope this will be helpful. If you have any further questions or concerns, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up