Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21744] to PDGFR beta (phospho Y751)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-PDGFR beta (phospho Y751) antibody [EPR21744]
See all PDGFR beta primary antibodies
DescriptionRabbit monoclonal [EPR21744] to PDGFR beta (phospho Y751)
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PDGFR beta aa 700-800 (phospho Y751). The exact sequence is proprietary.
Database link: P09619
- WB: IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes), NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) and C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab218534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 124 kDa).|
FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
- Information by UniProt
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
All lanes : Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534) at 1/1000 dilution
Lane 1 : Untreated IMR-90 (human lung fibroblast cell line) whole cell lysate
Lane 2 : IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
Lane 3 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
Lane 5 : Untreated C6 (rat glial tumor glial cell line) whole cell lysate
Lane 6 : C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 124 kDa
Observed band size: 124,190 kDa why is the actual band size different from the predicted?
Exposure times : Lanes 1-4: 26 seconds, Lanes 5 & 6: 3 minutes.
Blocking and dilution buffer: 1% BSA/TBST.
Expression of PDGFR beta (phospho Y751) is induced by PDGF-BB. The antibody recognises both the glycosylated and unglycosylated protein (PMID: 9307305).
Dot blot analysis of PDGFR beta (phospho Y751) labeled with ab218534 at 1/1000 dilution.
Lane 1: Human PDGFR beta (phospho Y751) peptide.
Lane 2: Human PDGFR beta non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab218534 has not yet been referenced specifically in any publications.