Recombinant
RabMAb

Recombinant Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)

Overview

  • Product name

    Anti-PDGFR beta (phospho Y751) antibody [EPR21744]
    See all PDGFR beta primary antibodies
  • Description

    Rabbit monoclonal [EPR21744] to PDGFR beta (phospho Y751)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PDGFR beta aa 700-800 (phospho Y751). The exact sequence is proprietary.
    Database link: P09619

  • Positive control

    • WB: IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes), NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) and C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 124 kDa).

Target

  • Function

    Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • Involvement in disease

    Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF Receptor beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

Images

  • All lanes : Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534) at 1/1000 dilution

    Lane 1 : Untreated IMR-90 (human lung fibroblast cell line) whole cell lysate
    Lane 2 : IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
    Lane 3 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
    Lane 5 : Untreated C6 (rat glial tumor glial cell line) whole cell lysate
    Lane 6 : C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124,190 kDa
    why is the actual band size different from the predicted?



    Exposure times : Lanes 1-4: 26 seconds, Lanes 5 & 6: 3 minutes.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Expression of PDGFR beta (phospho Y751) is induced by PDGF-BB. The antibody recognises both the glycosylated and unglycosylated protein (PMID: 9307305).

  • Dot blot analysis of PDGFR beta (phospho Y751) labeled with ab218534 at 1/1000 dilution.

    Lane 1: PDGFR beta (phospho Y751) peptide.
    Lane 2: PDGFR beta non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab218534 has not yet been referenced specifically in any publications.

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