Recombinant
RabMAb

Recombinant Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367)

Overview

  • Product name

    Anti-PDGFR beta (phospho Y857) antibody [EP2136Y]
    See all PDGFR beta primary antibodies
  • Description

    Rabbit monoclonal [EP2136Y] to PDGFR beta (phospho Y857)
  • Host species

    Rabbit
  • Specificity

    ab62367 detects PDGFR beta phosphorylated at Tyrosine 857.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    corresponding to Human PDGFR beta.

  • Positive control

    • NIH3T3 cell lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab62367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 123 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
    • Involvement in disease

      Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
      Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
      Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
      Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
      Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Beta platelet derived growth factor receptor antibody
      • Beta-type platelet-derived growth factor receptor antibody
      • CD 140B antibody
      • CD140 antigen-like family member B antibody
      • CD140b antibody
      • CD140b antigen antibody
      • IBGC4 antibody
      • IMF1 antibody
      • JTK12 antibody
      • OTTHUMP00000160528 antibody
      • PDGF R beta antibody
      • PDGF Receptor beta antibody
      • PDGF-R-beta antibody
      • PDGFR 1 antibody
      • PDGFR antibody
      • PDGFR beta antibody
      • PDGFR1 antibody
      • PDGFRB antibody
      • PGFRB_HUMAN antibody
      • Platelet derived growth factor receptor 1 antibody
      • Platelet derived growth factor receptor beta antibody
      • Platelet derived growth factor receptor beta polypeptide antibody
      see all

    Images

    • All lanes : Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367) at 1/1000 dilution

      Lane 1 : NIH3T3 cell lysates (untreated)
      Lane 2 : NIH3T3 cell lysates treated with PDGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit, HRP labeled, at 1/2000 dilution

      Predicted band size: 123 kDa
      Observed band size: 190 kDa
      why is the actual band size different from the predicted?

    References

    ab62367 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (BRAIN)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    30 µg
    Specification
    BRAIN
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Oct 27 2019

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