Overview

  • Product name

  • Assay type

    Quantitative
  • Species reactivity

    Reacts with: Cow, Human
  • Product overview

    250 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture PDH complex from heart mitochondria.


    Note: The immunocapture protocol for this kit requires Abcam reagent detergent lauryl maltoside (ab109857/MS910).


     

Properties

Images

  • PDH immunoprecipitation using antibody ab109866/MSP01c crosslinked to protein G-agarose beads as product ab109802

Protocols

References

This product has been referenced in:

  • Stenson BM  et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology 150:4104-13 (2009). Read more (PubMed: 19556420) »
  • Bonnet S  et al. A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell 11:37-51 (2007). Read more (PubMed: 17222789) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us. 
This kit size refers to the amount of antibody.  For example, the 250 ug kit contains 250 ug of antibody conjugated to 25 uL of beads (settled volume) suspended in a total volume of 400 uL. Thus the concentration of the antibody is 10 ug per uL of beads, or 0.625 ug per uL of whole suspension.  The 250 ug, 500 ug, and 750 ug sizes can capture ˜13 ug, ˜26 ug, or ˜39 ug respectively of PDH complex from heart mitochondria. 
For detecting Pyruvate Dehydrogenase E1-alpha, I would recommend either ab110330 or ab110334.  I have included all three sizes of the kit and both antibodies on the quote I sent you for your reference.  If you could let me know your shipping address and the products you are interested in, I will be happy to update the quote for you.

Read More

Answer

Thank you for contacting us.

I have had the opportunity to speak with the lab regarding this on your behalf. They recommend using ab110333 (MSP06) with rat samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH complex using IP reagents and protocol. The product page for ab110333 is located at:

https://www.abcam.com/Pyruvate-dehydrogenase-E2-E3bp-antibody-13G2AE2BH5-ab110333.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your previous email and I apologize that I'm just now getting around to reading it! I'll close any other correspondance so you won't be bothered again about this enquiry.

If you need any further information about these products please let me know and I'll be happy to help! Have a lovely day.

Read More

Answer

Thank you for contacting us with your question, and for your patience while I have been in touch with the lab about the specificity of this antibody.
My contact at the lab has responded:
"We did mass spectrometry and immunoblotting using this antibody and did not see any other branched chain dehydrogenases represented such as OGDH subunits.Enzymatically they are quite similar, but the primary sequences are not that similar. E.g. PDH E2 – OGDH E2 are only 29% identical."
I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help you. Have a great day!

Read More

Answer

Thank you for contacting us and for your interest in MSP01 (ab109802). I forwarded your email to the lab scientists at MitoSciences, and I received the following response: We do not know if the kinase and phosphatase are present, but if so then they are at low levels because we did not pick them up by mass spectrometry. We do know the enzyme is active.  Addition of ATP inhibits the enzyme activity which might suggest the presence of PDH kinases, however we do not see a corresponding increase in phosphorylation of PDH E1 alpha subunit. My thoughts are that they may be there in small amounts. I hope this information is helpful, but please let me know if you have any further questions.

Read More

Answer

Thank you for contacting us.

We haven't tested this kit with mouse samples so we unfortunately cannot provide any details. The kit has been optimized with cow and human samples so these samples are fully guaranteed.

We however recommend using ab110333 (MSP06) with mouse samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH complex using IP reagents and protocol. The product page for ab110333 is located at:

https://www.abcam.com/Pyruvate-dehydrogenase-E2-E3bp-antibody-13G2AE2BH5-ab110333.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up