Product namePDH Rodent Immunocapture Kit
Sample typeCell culture extracts, Tissue Extracts
Species reactivityReacts with: Mouse, Rat
The pyruvate dehydrogenase complex performs the decarboxylation of pyruvate into acetyl CoA, a critical function in metabolism, linking glycolysis and oxidative phosphorylation in mitochondria. The enzyme is composed of multiple copies of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide transacetylase (E2) and dihydrolipoamide dehydrogenase (E3). The E1 enzyme is a tetramer of two alpha (PDHA1) and two beta (PDHB) subunits.
Abcam ab133988 PDH Rodent Immunocapture Kit is suitable to immunocapture PDH complex from rodent tissue homogenates and whole cells lysates. Traditional immunoprecipitation methods usually result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results. The Abcam PDH immunocapture Kit resolves this issue by immobilize the PDH capture antibody onto protein G-agarose beads. The kit includes optimized buffers and reagents for sample preparation and PDH binding and recovery, which shorten the protocol and minimize handling and mixing.
Tested applicationsSuitable for: IPmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 200 µg 10 X Extraction Buffer 1 x 1ml 10 X PBS 1 x 10ml 10 X Wash Buffer 1 x 10ml Glycine Elution buffer 1 x 1ml Immunocapture Antibody Coupled Agarose Beads 1 x 100µl Neutralization buffer 1 x 1ml SDS Elution Buffer 1 x 1ml
- Pyruvate dehydrogenase
Our Abpromise guarantee covers the use of ab133988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
For all lanes: 10 µL antibody coated beads was mixed with 1 mg rodent sample and incubated for 3 hours. Captured PDH complex was eluted with SDS elution buffer and half volume of the eluted PDH complex was loaded on the gel. Gel was stained with Colloidal Coomassie Blue.
Lane 1: Ladder
Lane 2: PDH complex of RHH (rat heart homogenate)
Lane 3: PDH complex of MHH (mouse heart homogenate)
Lane 4: PDH complex of 1mg H4IIE (rat) whole cell lysate
Lane 5: PDH complex of 1mg 3T3 (mouse) whole cell lysate