RecombinantRabMAb

Recombinant Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12200] to PDHA1 (phospho S293) - BSA and Azide free
  • Suitable for: ELISA, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free
    See all PDHA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12200] to PDHA1 (phospho S293) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide aa 250-350 (phospho S293) (Cysteine residue). The exact sequence is proprietary.
    Database link: P08559

  • Positive control

    • IP: HEK-293T cell lysates. IHC-P: Human breast and colon tissue. Rat and mouse colon tissue.

  • General notes

    Ab227565 is the carrier-free version of ab177461. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227565 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab227565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • Function

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    • Pyruvate Dehydrogenase E1-alpha subunit antibody
    see all

Images

  • Immunoprecipitation - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    Immunoprecipitation - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

    ab177461 (purified) at 1:20 dilution (2µ) immunoprecipitating PDHA1 in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
    Lane 1 (input): HEK-293T whole cell lysate 10µg
    Lane 2 (+): ab177461 & HEK-293T whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab177461 in HEK-293T whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling PDHA1 with Purified ab177461 at 1:250 dilution (0.52 µ/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling PDHA1 with Purified ab177461 at 1:250 dilution (0.52 µ/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling PDHA1 with Purified ab177461 at 1:250 dilution (0.52 µ/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling PDHA1 with unpurified  ab177461 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177461).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

    This data was generated using ab177461, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PDHA1 with ab177461 at 1/50 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ELISA - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    ELISA - Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

    This data was generated using ab177461, the same antibody clone in a different buffer formulation.

    Serially diluted ab177461 was bound to immobilised human phospho peptide (S293) - or Control peptide (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.

  • Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)
    Anti-PDHA1 (phospho S293) antibody [EPR12200] - BSA and Azide free (ab227565)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (6)

Publishing research using ab227565? Please let us know so that we can cite the reference in this datasheet.

ab227565 has been referenced in 6 publications.

  • Zhang Y  et al. Regulation of hepatic pyruvate dehydrogenase phosphorylation in offspring glucose intolerance induced by intrauterine hyperglycemia. Oncotarget 8:15205-15212 (2017). PubMed: 28148899
  • Yang YL  et al. Ginsenoside Rg5 increases cardiomyocyte resistance to ischemic injury through regulation of mitochondrial hexokinase-II and dynamin-related protein 1. Cell Death Dis 8:e2625 (2017). PubMed: 28230856
  • Zhang Y  et al. Induction of Posttranslational Modifications of Mitochondrial Proteins by ATP Contributes to Negative Regulation of Mitochondrial Function. PLoS One 11:e0150454 (2016). Mouse . PubMed: 26930489
  • Barquissau V  et al. White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways. Mol Metab 5:352-65 (2016). PubMed: 27110487
  • Prabhu A  et al. Ras-mediated modulation of pyruvate dehydrogenase activity regulates mitochondrial reserve capacity and contributes to glioblastoma tumorigenesis. Neuro Oncol 17:1220-30 (2015). PubMed: 25712957
  • Cabanillas Stanchi KM  et al. Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma. Cancer Biol Ther 16:1353-63 (2015). PubMed: 26177922

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