Overview

  • Product name

  • Description

    Rabbit polyclonal to PDHB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PDHB aa 78-335.
    Database link: P11177

  • Positive control

    • A549, H1299, HCT116 and Mouse heart whole cell lysates; U87 xenograft tissue. ICC/IF: HepG2 and HeLA cells
  • General notes

    Keep as concentrated solution.

     This product was previously labelled as Pyruvate Dehydrogenase E1 beta subunit

     

Properties

Applications

Our Abpromise guarantee covers the use of ab126203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 1 µg/ml.

GR88682-3

Target

  • Function

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease

    Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Images

  • All lanes : Anti-PDHB antibody (ab126203) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • ICC/IF image of ab126203 stained HepG2 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126203, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Paraffin-embedded mouse intestine tissue labeling PDHB using ab126203 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Anti-PDHB antibody (ab126203) at 1/10000 dilution + Mouse heart whole cell lysate at 50 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • ab126203, at 1/500 dilution, staining PDHB in paraffin-embedded U87 xenograft tissue by Immunohistochemistry.

  • Anti-PDHB antibody (ab126203) at 1/5000 dilution + Rat heart lysate at 50 µg

    Predicted band size: 39 kDa

  • Paraffin-embedded mouse brain tissue labeling PDHB using ab126203 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Ice-cold methoanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PDHB using ab1262301 at 1/500 dilution (green) in ICC/IF.

    The nuclear counter stain is Hoechst 33342 (blue).

References

ab126203 has not yet been referenced specifically in any publications.

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