Key features and details
- Rabbit polyclonal to PDHB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PDHB antibody
See all PDHB primary antibodies
DescriptionRabbit polyclonal to PDHB
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Monkey, Xenopus tropicalis
Recombinant fragment corresponding to Human PDHB aa 1-314.
Database link: P11177
- A549, H1299, HCT116 whole cell lysate
This product was previously labelled as Pyruvate Dehydrogenase E1 beta subunit
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Involvement in diseaseDefects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
Cellular localizationMitochondrion matrix.
- Information by UniProt
- DKFZp564K0164 antibody
- mitochondrial antibody
- ODPB_HUMAN antibody
All lanes : Anti-PDHB antibody (ab154541) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling PDHB with ab154541 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PDHB with ab154541 at 1/500 dilution.
ab154541 has not yet been referenced specifically in any publications.