Overview

  • Product name

    Anti-PDHB antibody [EPR11096(B)]
    See all PDHB primary antibodies
  • Description

    Rabbit monoclonal [EPR11096(B)] to PDHB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human PDHB.
    Database link: P11177

  • Positive control

    • HepG2, HeLa, A375 and Human fetal heart lysates; Human colon and pancreas tissues; HepG2 and A375 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Pyruvate Dehydrogenase E1 beta subunit

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
    • Involvement in disease

      Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp564K0164 antibody
      • mitochondrial antibody
      • ODPB_HUMAN antibody
      • pdhB antibody
      • PDHBD antibody
      • PDHE1 B antibody
      • PDHE1-B antibody
      • PHE1B antibody
      • Pyruvate dehydrogenase (lipoamide) beta antibody
      • Pyruvate dehydrogenase E1 beta polypeptide antibody
      • Pyruvate dehydrogenase E1 component subunit beta antibody
      • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
      see all

    Images

    • All lanes : Anti-PDHB antibody [EPR11096(B)] (ab155953) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa

    • ab155953 showing +ve staining in Human thyroid gland carcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab155953 showing +ve staining in Human ovarian carcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab155953 showing +ve staining in Human heart.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab155953 showing +ve staining in Human gastric adenocarcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunofluorescent analysis of HepG2 cells, labeling PDHB with ab155953 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling PDHB with ab155953 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, labeling PDHB with ab155953 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow cytometric analysis of permeabilized A375 cells labeling PDHB with ab155953 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

    References

    ab155953 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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