Overview

  • Product name

    Anti-PDHB antibody [EPR11097(B)]
    See all PDHB primary antibodies
  • Description

    Rabbit monoclonal [EPR11097(B)] to PDHB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human PDHB.
    Database link: P11177

  • Positive control

    • HepG2, HeLa, A375 and Human fetal heart lysates; Human colon tissue; Human thyroid gland carcinoma tissue; HeLa cells.
  • General notes

     This product was previously labelled as Pyruvate Dehydrogenase E1 beta subunit

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease

    Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Images

  • All lanes : Anti-PDHB antibody [EPR11097(B)] (ab155996) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 35 kDa

  • ab155996 showing +ve staining in Human normal pancreas.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab155996 showing +ve staining in Human ovarian carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab155996 showing +ve staining in Human normal kidney.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab155996 showing +ve staining in Human ovarian carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PDHB with ab155996 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling PDHB with ab155996 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunofluorescence analysis of HeLa cells labeling PDHB with ab155996 at 1/100 dilution.

References

This product has been referenced in:

  • Groehler A  et al. Oxidative cross-linking of proteins to DNA following ischemia-reperfusion injury. Free Radic Biol Med 120:89-101 (2018). Rat . Read more (PubMed: 29540307) »
  • Jin X  et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). Read more (PubMed: 28487965) »
See all 3 Publications for this product

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