Product nameAnti-PDHX antibody
See all PDHX primary antibodies
DescriptionGoat polyclonal to PDHX
SpecificityThis antibody is expected to recognise all reported isoforms.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
- Human pancreas lysate. Human pancreas tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab31283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).|
|ELISA||Use at an assay dependent concentration.
Antibody detection limit dilution 1:32,000.
|IHC-P||Use a concentration of 1 - 3 µg/ml.|
FunctionRequired for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
Involvement in diseaseDefects in PDHX are the cause of pyruvate dehydrogenase E3-binding protein deficiency (PDHXD) [MIM:245349].
Sequence similaritiesBelongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex antibody
- Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody
- DLDBP antibody
Anti-PDHX antibody (ab31283) at 1 µg/ml (incubated for 1 hour) + Human Pancreas lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?