The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).
The observed molecular weight corresponds to the glycosylated form.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Highly expressed in pancreas (at protein level).
Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated.