The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Highly expressed in pancreas (at protein level).
Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated.
Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling PDI with ab180931 at 1/100 dilution (green) or Rabbit monoclonal IgG (red), followed with Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot - Anti-PDI antibody [EPR12212] (ab180931)
All lanes : Anti-PDI antibody [EPR12212] (ab180931) at 1/20000 dilution
Lane 1 : Human pancreas lysate Lane 2 : HeLa cell lysate Lane 3 : BxPC-3 cell lysate Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution