Overview

  • Product name
    Anti-PDI antibody [PDI-38H8]
    See all PDI primary antibodies
  • Description
    Mouse monoclonal [PDI-38H8] to PDI
  • Host species
    Mouse
  • Specificity
    ab30319 recognises Protein Disulphide Isomerase (PDI)
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Tissue/ cell preparation. Yeast nuclear protein preparations.

Properties

Applications

Our Abpromise guarantee covers the use of ab30319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
ICC/IF 1/250 - 1/2500.

Target

  • Function
    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • Tissue specificity
    Highly expressed in pancreas (at protein level).
  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Post-translational
    modifications
    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • Cellular localization
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Alternative names
    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

References

ab30319 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

Regrettably, we will not be able to guarantee the anti PDI antibodies cross reactivity towards P. pastoris species. The sequence analysis of ab30319 shows only 45% homology which is very less for antibody cross reactivity. I apologies for any inconvenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

 Dear technical support team: This customer has already purchased ab30319 (Anti-PDI antibody [PDI-38H8] - ER Marker), and after he conducted IHC with Saccharomyces cerevisiae sample, the result shows no signal, therefore he wants to ask for you help to offer any suggestion to him, and his experiment step as follow:   1) Order details: ˙Batch number ( Lot number): GR57411-1 .po: 952354 ˙ Abcam product code: ab30319   ˙ Antibody storage conditions (temperature/reconstitution etc) : -20℃ or 4℃for short time (no dilution) 2) Please describe the problem (high background, non-specific signal…etc). No signal   ) On what material are you testing the antibody in IHC/ICC? ˙ Species: S. cerevisiae ˙ What’s cell line or tissue: cell   4) Sample preparation: ˙Type of sample (Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed Formalin Fix cell solution ˙paraffin embedded sections, cells in culture, other:) : cell in culture ˙Sample preparation: add 100% formalin into culture solution directly to 10% working ˙ Positive control : no ˙Negative control : no   5) Fixation step ˙Yes or No : Yes ˙ If yes, Fixative agent and concentration: 10% working formalin ˙ Fixation time: 10~45min ˙ Fixation temperature :RT   6) Antigen retrieval method(including time, temperature etc.): no 7) Permeabilization method: ˙ Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? ˙Permeabilizing agent and concentration: Ice cold Methanol for about 4min and then acetone for about 10Sec Or add 0.03% trition X-100 in PBS for 10min   8) Blocking agent (eg BSA, serum…): ˙Concentration: 2%BSA ˙Blocking time : 30min                        ˙Blocking temperature: RT   9) ˙Endogenous peroxidases blocked? no ˙Endogenous biotins blocked? no 10) Primary antibody (If more than one was used, describe in “additional notes”) : ˙Species: mouse ˙Reacts against: Sc ˙At what dilution(s) have you tested this antibody:1:100~1:600 ˙Diluent buffer:  1%BSA in PBS ˙Incubation time: 1.5hrs or O/N ˙Incubation temperature: RT or 4℃ ˙What washing steps were done (which buffer, number of washes): PBS for 3 time (each time :just a few sec) 11)  Secondary antibody:       ˙Species: sheep ˙ Reacts against which species: mouse ˙At what dilution(s) have you tested this antibody: 1:400~1:800 ˙ Diluent buffer: 1% BSA inPBS ˙ Incubation time: 1hr ˙ What washing steps were done (which buffer, number of washes): PBS for 3 times (each time: just a few sec ˙ Fluorochrome or enzyme conjugate (eg: FITC, HRP, AP, biotin…etc): Cy3 ˙ Do you know whether the problems you are experiencing come from the secondary? Not 12) Signal amplification method (eg: ABC, LSAB, HRP polymer, TSE): No 13) Detection method (eg: DAB, BCIP/NBT …etc): Fluorescence 14)  How many times have you run this staining? About 4 times ˙Do you obtain the same results every time?  Yes ˙ What steps have you altered to try and optimize the use of this antibody? Dilution volume, permeabilizing type, and fix time Document attachment: Just no signal at zeiss 780 confocal   Could you please help this customer to solve the problem? Thanks for your kindly help Best regards

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will provide us with vital information for our monitoring of product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial. I apologize for the inconvenience and am pleased to offer you a free of charge replacement or credit note in compensation. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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