Overview

  • Product name
    Anti-PDI1 antibody [38H8]
  • Description
    Mouse monoclonal [38H8] to PDI1
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Cell membrane preparation (Saccharomyces cerevisiae)

Properties

Applications

Our Abpromise guarantee covers the use of ab4644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000. (gives an ER staining pattern on yeast cells).
WB Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 58.2 kDa). Suggested working dilution of 1/5000 (see PMID: 17051412).

Target

  • Relevance
    PDI1 is a protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds.
  • Database links
    • Alternative names
      • MFP1 antibody
      • PDI1 antibody
      • Pdi1p antibody
      • Protein disulfide isomerase antibody
      • Thioredoxin related glycoprotein 1 antibody
      • TRG1 antibody
      see all

    References

    This product has been referenced in:
    • Delic M  et al. Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast. Free Radic Biol Med 52:2000-12 (2012). WB . Read more (PubMed: 22406321) »
    • Damasceno LM  et al. Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris. Appl Microbiol Biotechnol 74:381-9 (2007). Read more (PubMed: 17051412) »

    See all 2 Publications for this product

    Publishing research using ab4644? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    According to a datasheet of ab4644, cellular localisation of PDI1 is ER and nuclear envelope. Have you ascertain the localisation in immune electron microscopy or western blotting of cytosolic fractions?

    Thank you for your enquiry. I can confirm this antibody was screened by immunocytochemistry, selecting for various clones that stained yeast cells in an interesting and robust fashion. This particular antibody stains ER and the nuclear membrane, wh...

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    Thanks for your prompt reply.
    Find attached please the protein sequences (FASTA format) of P.
    pastoris PDI and KAR2 and the locus identification of each protein
    with which you can look for the other sequence format in Uniprot, for
    in...

    Read More

    Thank you for contacting us.

    Regrettably, we will not be able to guarantee the anti PDI antibodies cross reactivity towards P. pastoris species. The sequence analysis of ab30319 shows only 45% homology which is very less for antibody cross rea...

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    Dear Sir/Madam Thank you for your reply. However I must say I am rather disappointed, as my expectations after the first call were not met. Also, our lab recently bought several other antibodies from you (therein ab6160 and ab8224, Ref. ...

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    Thank you for your email. I apologize for your disappointment. I will try to explain what could have happen. The antibodies ab4644 and ab34772 were dispatched on 4th july and were delivered on 7th July. I am not sure if the person who received t...

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    I was wondering if you have any references for papers that have used this antibody for immunofluorescent studies Marcus

    Unfortunately, we are not aware of any publications that feature the use of this antibody.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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