Key features and details
- Goat polyclonal to PDIA2/PDIP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PDIA2/PDIP antibody
See all PDIA2/PDIP primary antibodies
DescriptionGoat polyclonal to PDIA2/PDIP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
- WB: Human, mouse and rat pancreas lysates. IHC-P: Human pancreas tissue.
This product was previously labelled as PDIA2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223520 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Tissue specificityHighly expressed in pancreas (at protein level).
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
modificationsThe disulfide-linked homodimer exhibits an enhanced chaperone activity.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- Pancreas specific protein disulfide isomerase antibody
- Pancreas-specific protein disulfide isomerase antibody
- Pancreatic protein disulfide isomerase antibody
Paraffin-embedded human pancreas tissue stained for PDIA2/PDIP using ab223520 at 3.8 µg/mL in immunohistochemical analysis, followed by AP-staining.
All lanes : Anti-PDIA2/PDIP antibody (ab223520) at 0.1 µg/ml
Lane 1 : Human pancreas lysate (in RIPA buffer)
Lane 2 : Mouse pancreas lysate (in RIPA buffer)
Lane 3 : Rat pancreas lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223520 has not yet been referenced specifically in any publications.