Overview

  • Product name

  • Description

    Goat polyclonal to PDIA2/PDIP
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Orangutan
  • Immunogen

    Tissue, cells or virus corresponding to Human PDIA2/PDIP aa 477-489 (internal sequence) (Cysteine residue). NP_006840.2.
    Sequence:

    C-EYKSTRDLETFSK


    Database link: Q13087

  • Positive control

    • WB: Human, mouse and rat pancreas lysates. IHC-P: Human pancreas tissue.
  • General notes

     This product was previously labelled as PDIA2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab223520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • Tissue specificity

    Highly expressed in pancreas (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Post-translational
    modifications

    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

Images

  • Paraffin-embedded human pancreas tissue stained for PDIA2/PDIP using ab223520 at 3.8 µg/mL in immunohistochemical analysis, followed by AP-staining.

  • All lanes : Anti-PDIA2/PDIP antibody (ab223520) at 0.1 µg/ml

    Lane 1 : Human pancreas lysate (in RIPA buffer)
    Lane 2 : Mouse pancreas lysate (in RIPA buffer)
    Lane 3 : Rat pancreas lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223520 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223520.
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