Product nameAnti-PDIA2/PDIP antibody [EPR12212]
See all PDIA2/PDIP primary antibodies
DescriptionRabbit monoclonal [EPR12212] to PDIA2/PDIP
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PDIA2/PDIP aa 400-500. The exact sequence is proprietary.
Database link: Q13087
- Human pancreas, HeLa, BxPC-3 and HepG2 cell lysates; HeLa cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Tissue specificityHighly expressed in pancreas (at protein level).
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
modificationsThe disulfide-linked homodimer exhibits an enhanced chaperone activity.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- Pancreas specific protein disulfide isomerase antibody
- Pancreas-specific protein disulfide isomerase antibody
- Pancreatic protein disulfide isomerase antibody
Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling PDIA2/PDIP with ab180931 at 1/100 dilution (green) or Rabbit monoclonal IgG (red), followed with Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
All lanes : Anti-PDIA2/PDIP antibody [EPR12212] (ab180931) at 1/20000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 58 kDa
ab180931 has not yet been referenced specifically in any publications.