Overview

  • Product name

  • Description

    Rabbit polyclonal to PDIA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human PDIA5 aa 378-506. Expressed in E.coli.
    Sequence:

    WMQNPEAPPPPEPTWEEQQTSVLHLVGDNFRETLKKKKHTLVMFYAPWCP HCKKVIPHFTATADAFKDDRKIACAAVDCVKDKNQDLCQQEAVKGYPTFH YYHYGKFAEKYDSDRTELGFTNYIRALRE


    Database link: Q14554

  • Positive control

    • WB: Recombinant human PDIA5 protein, pig liver tissue lysate and HeLa and A431 cell lysates. IHC-P: Human liver tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 60 kDa.

Target

Images

  • Anti-PDIA5 antibody (ab232979) at 2 µg/ml + Recombinant human PDIA5 protein

    Predicted band size: 60 kDa

  • Anti-PDIA5 antibody (ab232979) at 2 µg/ml + Pig liver lysate

    Predicted band size: 60 kDa

  • Anti-PDIA5 antibody (ab232979) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 60 kDa

  • Anti-PDIA5 antibody (ab232979) at 2 µg/ml + A431 (human epidermoid carcinoma cell line) cell lysate

    Predicted band size: 60 kDa

  • Formalin-fixed, paraffin-embedded human liver tissue stained for PDIA5 using ab232979 at 30 μg/ml in immunohistochemical analysis. DAB staining.

References

ab232979 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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