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    pdia6-antibody-ab11432.pdf

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Signal Transduction Protein Trafficking ER Proteins
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Anti-PDIA6 antibody (ab11432)

  • Datasheet
Submit a review Submit a question References (8)

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Western blot - Anti-PDIA6 antibody (ab11432)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)

Key features and details

  • Rabbit polyclonal to PDIA6
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PDIA6 antibody
    See all PDIA6 primary antibodies
  • Description

    Rabbit polyclonal to PDIA6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human PDIA6 aa 476-492.
    Sequence:

    DIDLSDVELDDLGKDEL


    (Peptide available as ab11432)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Anti-PDIA6 antibody (ab11432)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PDIA6 protein (ab101048)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Rat
Hamster
Xenopus laevis
Zebrafish
Application Abreviews Notes
ICC/IF
1/250.
WB
1/250. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432).
Notes
ICC/IF
1/250.
WB
1/250. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432).

Target

  • Function

    May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
  • Tissue specificity

    Expressed in platelets (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession Q15084 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10130 Human
    • Entrez Gene: 286906 Rat
    • Omim: 611099 Human
    • SwissProt: Q15084 Human
    • SwissProt: Q63081 Rat
    • Unigene: 212102 Human
    • Unigene: 580464 Human
    • Unigene: 2685 Rat
    • Alternative names

      • Endoplasmic reticulum protein 5 antibody
      • ER protein 5 antibody
      • ERp5 antibody
      • P5 antibody
      • Pdia6 antibody
      • PDIA6_HUMAN antibody
      • Protein disulfide isomerase A6 antibody
      • Protein disulfide isomerase associated 6 antibody
      • Protein disulfide isomerase family A member 6 antibody
      • Protein disulfide isomerase P5 antibody
      • Protein disulfide isomerase related protein antibody
      • Protein disulfide-isomerase A6 antibody
      • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
      • Thioredoxin domain containing protein 7 antibody
      • Thioredoxin domain-containing protein 7 antibody
      • TXNDC7 antibody
      see all

    Images

    • Western blot - Anti-PDIA6 antibody (ab11432)
      Western blot - Anti-PDIA6 antibody (ab11432)

      Western blot of PDIA6 in HT 1080 cell lysate using ab11432. 

    • Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)
      Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)
      ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab11432? Please let us know so that we can cite the reference in this datasheet.

    ab11432 has been referenced in 8 publications.

    • Lee HY  et al. Phosphatidylinositol 3-kinase-d controls endoplasmic reticulum membrane fluidity and permeability in fungus-induced allergic inflammation in mice. Br J Pharmacol 177:1556-1567 (2020). PubMed: 31713846
    • Pavlenko E  et al. Functional Characterization of Colon Cancer-Associated Mutations in ADAM17: Modifications in the Pro-Domain Interfere with Trafficking and Maturation. Int J Mol Sci 20:N/A (2019). PubMed: 31060243
    • Cabron AS  et al. Structural and Functional Analyses of the Shedding Protease ADAM17 in HoxB8-Immortalized Macrophages and Dendritic-like Cells. J Immunol 201:3106-3118 (2018). PubMed: 30355783
    • Mazzulli JR  et al. Activation of ß-Glucocerebrosidase Reduces Pathological a-Synuclein and Restores Lysosomal Function in Parkinson's Patient Midbrain Neurons. J Neurosci 36:7693-706 (2016). PubMed: 27445146
    • Zingoni A  et al. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol 195:736-48 (2015). PubMed: 26071561
    • Groenendyk J  et al. Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis. Sci Signal 7:ra54 (2014). WB . PubMed: 24917591
    • Wang X  et al. An six-amino acid motif in the alpha3 domain of MICA is the cancer therapeutic target to inhibit shedding. Biochem Biophys Res Commun 387:476-81 (2009). WB ; Human . PubMed: 19615970
    • Viklund IM  et al. Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel disease: a potential marker gene for ulcerative colitis. Int J Colorectal Dis 23:921-30 (2008). PubMed: 18654788

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