Anti-PDIA6 antibody (ab11432)
Key features and details
- Rabbit polyclonal to PDIA6
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDIA6 antibody
See all PDIA6 primary antibodies -
Description
Rabbit polyclonal to PDIA6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PDIA6 aa 476-492.
Sequence:DIDLSDVELDDLGKDEL
(Peptide available asab11432)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Rat
Hamster
Xenopus laevis
Zebrafish
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/250.
|
|
| WB |
1/250. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432).
|
| Notes |
|---|
|
ICC/IF
1/250. |
|
WB
1/250. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432). |
Target
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Function
May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. -
Tissue specificity
Expressed in platelets (at protein level). -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 10130 Human
- Entrez Gene: 286906 Rat
- Omim: 611099 Human
- SwissProt: Q15084 Human
- SwissProt: Q63081 Rat
- Unigene: 212102 Human
- Unigene: 580464 Human
- Unigene: 2685 Rat
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Alternative names
- Endoplasmic reticulum protein 5 antibody
- ER protein 5 antibody
- ERp5 antibody
see all
Images
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Western blot - Anti-PDIA6 antibody (ab11432)
Western blot of PDIA6 in HT 1080 cell lysate using ab11432.
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Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (8)
ab11432 has been referenced in 8 publications.
- Lee HY et al. Phosphatidylinositol 3-kinase-d controls endoplasmic reticulum membrane fluidity and permeability in fungus-induced allergic inflammation in mice. Br J Pharmacol 177:1556-1567 (2020). PubMed: 31713846
- Pavlenko E et al. Functional Characterization of Colon Cancer-Associated Mutations in ADAM17: Modifications in the Pro-Domain Interfere with Trafficking and Maturation. Int J Mol Sci 20:N/A (2019). PubMed: 31060243
- Cabron AS et al. Structural and Functional Analyses of the Shedding Protease ADAM17 in HoxB8-Immortalized Macrophages and Dendritic-like Cells. J Immunol 201:3106-3118 (2018). PubMed: 30355783
- Mazzulli JR et al. Activation of ß-Glucocerebrosidase Reduces Pathological a-Synuclein and Restores Lysosomal Function in Parkinson's Patient Midbrain Neurons. J Neurosci 36:7693-706 (2016). PubMed: 27445146
- Zingoni A et al. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol 195:736-48 (2015). PubMed: 26071561
- Groenendyk J et al. Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis. Sci Signal 7:ra54 (2014). WB . PubMed: 24917591
- Wang X et al. An six-amino acid motif in the alpha3 domain of MICA is the cancer therapeutic target to inhibit shedding. Biochem Biophys Res Commun 387:476-81 (2009). WB ; Human . PubMed: 19615970
- Viklund IM et al. Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel disease: a potential marker gene for ulcerative colitis. Int J Colorectal Dis 23:921-30 (2008). PubMed: 18654788
