Product nameAnti-PDIA6 antibody
See all PDIA6 primary antibodies
DescriptionRabbit polyclonal to PDIA6
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human PDIA6 aa 390-440. The exact sequence is proprietary. (NP_005733.1).
Database link: Q15084
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225993 was affinity purified using an epitope specific to PDIA6 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 48 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
Tissue specificityExpressed in platelets (at protein level).
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Endoplasmic reticulum protein 5 antibody
- ER protein 5 antibody
- ERp5 antibody
All lanes : Anti-PDIA6 antibody (ab225993) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
PDIA6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab225993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225993 at 0.1 µg/ml.
Lane 1: ab225993IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PDIA6 using ab225993 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
ab225993 has not yet been referenced specifically in any publications.