Overview

  • Product name

  • Description

    Rabbit polyclonal to PDIA6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Orangutan
  • Immunogen

    Synthetic peptide within Human PDIA6 aa 250-300. The exact sequence is proprietary. (NP_005733.1).
    Database link: Q15084

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IHC-P: Human colon carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226840 was affinity purified using an epitope specific to PDIA6 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
    • Tissue specificity

      Expressed in platelets (at protein level).
    • Sequence similarities

      Belongs to the protein disulfide isomerase family.
      Contains 2 thioredoxin domains.
    • Cellular localization

      Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • Endoplasmic reticulum protein 5 antibody
      • ER protein 5 antibody
      • ERp5 antibody
      • P5 antibody
      • Pdia6 antibody
      • PDIA6_HUMAN antibody
      • Protein disulfide isomerase A6 antibody
      • Protein disulfide isomerase associated 6 antibody
      • Protein disulfide isomerase family A member 6 antibody
      • Protein disulfide isomerase P5 antibody
      • Protein disulfide isomerase related protein antibody
      • Protein disulfide-isomerase A6 antibody
      • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
      • Thioredoxin domain containing protein 7 antibody
      • Thioredoxin domain-containing protein 7 antibody
      • TXNDC7 antibody
      see all

    Images

    • All lanes : Anti-PDIA6 antibody (ab226840) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 48 kDa


      Exposure time: 30 seconds


      Lysates were prepared using NETN lysis buffer.

    • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PDIA6 using ab226840 at 1/5000 dilution in immunohistochemical analysis.

      Detection: DAB staining. Counterstain: Hematoxylin (blue).

    References

    ab226840 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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