Overview

  • Product name

    Anti-PDIA6 antibody [EPR10132(B)]
    See all PDIA6 primary antibodies
  • Description

    Rabbit monoclonal [EPR10132(B)] to PDIA6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human PDIA6 (Q15084).

  • Positive control

    • Human fetal kidney tissue lysate; K562; HepG2 and HT 1080 cell lysates; HepG2 cells; HT 1080 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10132(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
    • Tissue specificity

      Expressed in platelets (at protein level).
    • Sequence similarities

      Belongs to the protein disulfide isomerase family.
      Contains 2 thioredoxin domains.
    • Cellular localization

      Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • Endoplasmic reticulum protein 5 antibody
      • ER protein 5 antibody
      • ERp5 antibody
      • P5 antibody
      • Pdia6 antibody
      • PDIA6_HUMAN antibody
      • Protein disulfide isomerase A6 antibody
      • Protein disulfide isomerase associated 6 antibody
      • Protein disulfide isomerase family A member 6 antibody
      • Protein disulfide isomerase P5 antibody
      • Protein disulfide isomerase related protein antibody
      • Protein disulfide-isomerase A6 antibody
      • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
      • Thioredoxin domain containing protein 7 antibody
      • Thioredoxin domain-containing protein 7 antibody
      • TXNDC7 antibody
      see all

    Images

    • All lanes : Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HT 1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 48 kDa

    • Immunofluorescence analysis of HepG2 cells, labeling PDIA6 using ab154820 at 1/250 dilution.
    • Flow cytometric analysis of permeabilized HT 1080 cells labeling PDIA6 using ab154820 at 1/100 dilution (red), or rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Bell PA  et al. Mesencephalic astrocyte-derived neurotropic factor is an important factor in chondrocyte ER homeostasis. Cell Stress Chaperones 24:159-173 (2019). Read more (PubMed: 30543055) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (brain section)
    Specification
    brain section
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 02 2018

    Application
    Western blot
    Sample
    Horse Tissue lysate - whole (Whole liver extract)
    Gel Running Conditions
    Non-reduced Denaturing (12 %)
    Loading amount
    60 µg
    Specification
    Whole liver extract
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted May 23 2017

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (HeLa, RPE-1)
    Specification
    HeLa, RPE-1
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Jul 08 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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