Recombinant Anti-PDIA6 antibody [EPR10132(B)] (HRP) (ab201733)


  • Product name

    Anti-PDIA6 antibody [EPR10132(B)] (HRP)
    See all PDIA6 primary antibodies
  • Description

    Rabbit monoclonal [EPR10132(B)] to PDIA6 (HRP)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PDIA6 aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: Q15084

  • Positive control

    • WB: Human fetal kidney tissue lysate; HepG2 and HT1080 whole cell lysates. IHC-P: FFPE human normal bladder tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab201733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.


WB 1/5000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 48 kDa).


  • Function

    May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
  • Tissue specificity

    Expressed in platelets (at protein level).
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endoplasmic reticulum protein 5 antibody
    • ER protein 5 antibody
    • ERp5 antibody
    • P5 antibody
    • Pdia6 antibody
    • PDIA6_HUMAN antibody
    • Protein disulfide isomerase A6 antibody
    • Protein disulfide isomerase associated 6 antibody
    • Protein disulfide isomerase family A member 6 antibody
    • Protein disulfide isomerase P5 antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A6 antibody
    • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
    • Thioredoxin domain containing protein 7 antibody
    • Thioredoxin domain-containing protein 7 antibody
    • TXNDC7 antibody
    see all


  • IHC image of PDIA6 staining in a section of formalin-fixed paraffin-embedded human normal bladder*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201733, 1/200 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-PDIA6 antibody [EPR10132(B)] (HRP) (ab201733) at 1/5000 dilution

    Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48,50 kDa
    why is the actual band size different from the predicted?

    Exposure time: 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201733 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.


ab201733 has not yet been referenced specifically in any publications.

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