Overview

  • Product name

    Anti-PDILT antibody
  • Description

    Rabbit polyclonal to PDILT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Mouse PDILT aa 550-563 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    IRKPEEPERRKETA


    Database link: Q9DAN1

    Synthetic peptide corresponding to Mouse PDILT aa 571-584 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence: QPKEQPKPERKLEV
    Database link: Q9DAN1

  • Positive control

    • Testes extracts from wild-type and PDILT single knock-out mice; mouse testis tissue extract; mouse testicular section.

Properties

Applications

Our Abpromise guarantee covers the use of ab191851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 68 kDa.
IP 1/100.
IHC-Fr 1/100 - 1/500.
IHC-P 1/100 - 1/500.
ICC/IF 1/100.

Target

  • Function

    Probable redox-inactive chaperone involved in spermatogenesis.
  • Tissue specificity

    Testis-specific.
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 1 thioredoxin domain.
  • Domain

    The thioredoxin domain lacks the conserved redox-active Cys at position 417 which is replaced by a Ser residue, suggesting that it lacks thioredoxin activity.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1700007B13Rik antibody
    • PDIA7 antibody
    • Pdilt antibody
    • PDILT_HUMAN antibody
    • Protein disulfide isomerase family A member 7 antibody
    • Protein disulfide isomerase like testis expressed antibody
    • Protein disulfide-isomerase-like protein of the testis antibody
    • RGD1307822 antibody
    see all

Images

  • Immunohistochemical staining of paraffin embedded mouse testis tissue using ab191851 at a dilution of 1/1000. The secondary antibody was used at a dilution of 1/1000 and the sample was counter stained with DAPI.

  • All lanes : Anti-PDILT antibody (ab191851) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Mouse sperm cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP anti-rabbit IgG at 1/10000 dilution

    Predicted band size: 68 kDa

  • All lanes : Anti-PDILT antibody (ab191851) at 1/1000 dilution

    Lane 1 : mouse brain tissue lysate at 30 µg
    Lane 2 : mouse lung tissue lysate at 30 µg
    Lane 3 : mouse heart tissue lysate at 30 µg
    Lane 4 : mouse thymus tissue lysate at 30 µg
    Lane 5 : mouse liver tissue lysate at 30 µg
    Lane 6 : mouse spleen tissue lysate at 30 µg
    Lane 7 : mouse kidney tissue lysate at 30 µg
    Lane 8 : mouse muscle tissue lysate at 30 µg
    Lane 9 : mouse ovary tissue lysate at 30 µg
    Lane 10 : mouse testis tissue lysate at 30 µg
    Lane 11 : mouse sperm lysate at 10 µg

    Predicted band size: 68 kDa

  • All lanes : Anti-PDILT antibody (ab191851) at 1/1000 dilution

    Lane 1 : Testes extracts from wild-type (+/+) mice
    Lane 2 : Testes extracts from PDILT single knock-out mice (+/-)
    Lane 3 : Testes extracts from PDILT double knock-out mice (-/-)

    Predicted band size: 68 kDa



    Lower panel: detection of alpha tubulin was used as loading control.

  • Immunoprecipitation of PDILT protein from mouse testis extracts. Extracts (100 μg) of wild-type (+/+) and PDILT (-/-) knock out mouse testes were immunoprecipitated with ab191851 at 1/100 dilution and the precipitates were analyzed by western blotting using ab191851 at 1/1000 dilution.

    1. Input. 2. Wild-type. 3. PDILT (-/-) knock out

  • Immunofluorescent analysis of paraformaldehyde-fixed, cryopreserved in graded 10-30% sucrose, and embedded in a TissueTek OCT compound mouse testicular sections labeling PDILT with ab191851 at 1/100 and as a secondary antibody, Alexa Fluor 546 conjugated anti-rabbit IgG antibody was used. Image at the top: mouse spermatocyte; image at the bottom mouse spermatid.

     

References

ab191851 has not yet been referenced specifically in any publications.

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