Recombinant Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19727] to PDK1 + PDK2 + PDK4
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727]
See all PDK1 + PDK2 + PDK4 primary antibodies -
Description
Rabbit monoclonal [EPR19727] to PDK1 + PDK2 + PDK4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse PDK4, PDK1 and PDK2 recombinant proteins; mouse skeletal muscle, heart and liver lysates; C2C12 and HepG2 whole cell lysates; human and rat heart lysates; gastrocnemius muscle lysate from un-starved mouse and mouse starved for 72 hours; heart lysate from un-starved mouse and mouse starved for 72 hours. IP: Mouse skeletal muscle whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19727 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
IP
1/30. |
Images
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All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/10000 dilution
Lane 1 : Mouse PDK4 recombinant protein
Lane 2 : Mouse PDK1 recombinant protein
Lane 3 : Mouse PDK2 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.
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All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : Human heart lysate
Lane 7 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 10 seconds; Lane 5-6:3 minutes; Lane 7: 2 seconds.
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PDK1+PDK2+PDK4 was immunoprecipitated from 0.35 mg of Mouse skeletal muscle whole cell lysate with ab208187 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab208187 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse skeletal muscle whole cell lysate 10µg (Input).
Lane 2: ab208187 IP in Mouse skeletal muscle whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208187 in Mouse skeletal muscle whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab208187 has not yet been referenced specifically in any publications.