Recombinant
RabMAb

Recombinant Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)

Overview

  • Product name

    Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727]
    See all PDK1 + PDK2 + PDK4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19727] to PDK1 + PDK2 + PDK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse PDK1 + PDK2 + PDK4 aa 1 to the C-terminus. The exact sequence is proprietary. Corresponding to PDK4
    Database link: O70571

  • Positive control

    • WB: Mouse PDK4, PDK1 and PDK2 recombinant proteins; mouse skeletal muscle, heart and liver lysates; C2C12 and HepG2 whole cell lysates; human and rat heart lysates; gastrocnemius muscle lysate from un-starved mouse and mouse starved for 72 hours; heart lysate from un-starved mouse and mouse starved for 72 hours. IP: Mouse skeletal muscle whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19727
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab208187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IP 1/30.

Images

  • All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/10000 dilution

    Lane 1 : Mouse PDK4 recombinant protein
    Lane 2 : Mouse PDK1 recombinant protein
    Lane 3 : Mouse PDK2 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.

  • All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : Human heart lysate
    Lane 7 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 10 seconds; Lane 5-6:3 minutes; Lane 7: 2 seconds.

  • PDK1+PDK2+PDK4 was immunoprecipitated from 0.35 mg of Mouse skeletal muscle whole cell lysate with ab208187 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab208187 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse skeletal muscle whole cell lysate 10µg (Input).

    Lane 2: ab208187 IP in Mouse skeletal muscle whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208187 in Mouse skeletal muscle whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

ab208187 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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