Anti-PDK1 antibody (ab90444)
Key features and details
- Rabbit polyclonal to PDK1
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Dog, Human, Pig, African green monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PDK1 antibody
See all PDK1 primary antibodies -
Description
Rabbit polyclonal to PDK1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Dog, Human, Pig, African green monkey -
Immunogen
Synthetic peptide corresponding to Human PDK1 conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 49 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 49 kDa. |
Target
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Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Expressed predominantly in the heart. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 5163 Human
- Entrez Gene: 228026 Mouse
- Entrez Gene: 100286871 Pig
- Entrez Gene: 116551 Rat
- Omim: 602524 Human
- SwissProt: Q15118 Human
- SwissProt: Q8BFP9 Mouse
- SwissProt: Q63065 Rat
see all -
Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab90444 has been referenced in 8 publications.
- Wang H et al. PD-L1, PDK-1 and p-Akt are correlated in patients with papillary thyroid carcinoma. Adv Clin Exp Med 29:785-792 (2020). PubMed: 32750756
- Hong SM et al. Lactic acidosis caused by repressed lactate dehydrogenase subunit B expression down-regulates mitochondrial oxidative phosphorylation via the pyruvate dehydrogenase (PDH)-PDH kinase axis. J Biol Chem 294:7810-7820 (2019). PubMed: 30923124
- Pendleton AL et al. Increased pyruvate dehydrogenase activity in skeletal muscle of growth-restricted ovine fetuses. Am J Physiol Regul Integr Comp Physiol 317:R513-R520 (2019). PubMed: 31314546
- Shibao S et al. Metabolic heterogeneity and plasticity of glioma stem cells in a mouse glioblastoma model. Neuro Oncol 20:343-354 (2018). PubMed: 29016888
- Gudiksen A et al. Training state and fasting-induced PDH regulation in human skeletal muscle. Pflugers Arch N/A:N/A (2018). PubMed: 29946822
- Bertholdt L et al. Impact of skeletal muscle IL-6 on regulation of liver and adipose tissue metabolism during fasting. Pflugers Arch 470:1597-1613 (2018). PubMed: 30069669
- Gudiksen A et al. Muscle interleukin-6 and fasting-induced PDH regulation in mouse skeletal muscle. Am J Physiol Endocrinol Metab 312:E204-E214 (2017). PubMed: 28028037
- Gudiksen A et al. Lack of Skeletal Muscle IL-6 Affects Pyruvate Dehydrogenase Activity at Rest and during Prolonged Exercise. PLoS One 11:e0156460 (2016). WB . PubMed: 27327080