Recombinant Anti-PDK1 antibody [EPR13009] (ab175190)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-PDK1 antibody [EPR13009]
See all PDK1 primary antibodies -
DescriptionRabbit monoclonal [EPR13009] to PDK1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human PDK1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15118 -
Positive control
- LNCaP and Human fetal heart lysate
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General notes
This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR13009
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 49 kDa. | |
| IP | 1/10 - 1/100. |
Target
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FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
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Tissue specificityExpressed predominantly in the heart.
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Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localizationMitochondrion matrix.
- Information by UniProt
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Database links
- Entrez Gene: 5163 Human
- Entrez Gene: 228026 Mouse
- Entrez Gene: 116551 Rat
- Omim: 602524 Human
- SwissProt: Q15118 Human
- SwissProt: Q8BFP9 Mouse
- SwissProt: Q63065 Rat
- Unigene: 470633 Human
see all -
Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
see all
Images
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![Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)](https://www.abcam.com/ps/products/175/ab175190/Images/ab175190-259100-ab175190KOWB.jpg)
Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PDK1 knockout HAP1 cell lysate (20 µg)
Lane 3: Human fetal heart lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab175190 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab175190 was shown to specifically recognize PDK1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PDK1 knockout samples were examined. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. ab175190 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)](https://www.abcam.com/ps/products/175/ab175190/Images/ab175190-201520-ab1751901.jpg)
Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)All lanes : Anti-PDK1 antibody [EPR13009] (ab175190) at 1/1000 dilution
Lane 1 : LNCaP lysates
Lane 2 : Human fetal heart lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa -
![Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (ab175190)](https://www.abcam.com/ps/products/175/ab175190/Images/ab175190-201519-ab1751902.jpg)
Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (ab175190)Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate (lane 1) labeling PDK1 with ab175190 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Datasheets and documents
References
This product has been referenced in:
- Wang F et al. Hypoxia regulates sumoylation pathways in intervertebral disc cells: implications for hypoxic adaptations. Osteoarthritis Cartilage 24:1113-24 (2016). Read more (PubMed: 26826302) »
