Recombinant
RabMAb

Recombinant Anti-PDK1 antibody [SP270] - C-terminal (ab227682)

Overview

  • Product name

    Anti-PDK1 antibody [SP270] - C-terminal
    See all PDK1 primary antibodies
  • Description

    Rabbit monoclonal [SP270] to PDK1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PDK1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q15118

  • Positive control

    • IHC-P: Human kidney, liver, stomach, heart, pancreas, testis and liver hepatocellular carcinoma tissues.
  • General notes

     This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity

    Expressed predominantly in the heart.
  • Sequence similarities

    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
    • HGNC:8809 antibody
    • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
    • PDH kinase 1 antibody
    • Pdk1 antibody
    • PDK1_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoform 1 antibody
    • Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for PDK1 using ab227682 at 1/100 dilution  in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human heart tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreas tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human testis tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

References

ab227682 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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