Overview

  • Product name

    Anti-PDK2 antibody [3F2D7]
    See all PDK2 primary antibodies
  • Description

    Mouse monoclonal [3F2D7] to PDK2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human PDK2 aa 178-404. Expressed in E. Coli.
    Sequence:

    GST NPAHPKHIGS IDPNCNVSEV VKDAYDMAKL LCDKYYMASP DLEIQEINAA NSKQPIHMVY VPSHLYHMLF ELFKNAMRAT VESHESSLIL PPIKVMVALG EEDLSIKMSD RGGGVPLRKI ERLFSYMYST APTPQPGTGG TPLAGFGYGL PISRLYAKYF QGDLQLFSME GFGTDAVIYL KALSTDSVER LPVYNKSAWR HYQTIQEAGD WCVPSTEPKN TSTY


    Database link: Q15119

  • Positive control

    • A431 cells; Human recombinant PDK2 (aa178-404) protein; Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate; Jurkat , C6), COS7, K562 and A431 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab201964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 46 kDa.
Flow Cyt 1/200 - 1/400.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity

    Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
  • Sequence similarities

    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody
    • mitochondrial antibody
    • PDHK2 antibody
    • PDK2 antibody
    • PDK2_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoform 2 antibody
    • Pyruvate dehydrogenase kinase, isozyme 2 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: PDK2 knockout HAP1 cell lysate (40 µg)
    Lane 3: SHSY5Y cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab201964 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab201964 was shown to recognize PDK2 when PDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and PDK2 knockout samples were subjected to SDS-PAGE.  Ab201964 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) ab216772 and IRDye® 680 Goat anti-Rabbit IgG (H + L) ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Flow cytometric analysis of A431 cells labeling PDK2 with ab201964 at 1/200 dilution (green). Negative control (red).

  • All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : A431 cell lysate

    Predicted band size: 46 kDa

  • All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 46 kDa

  • Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution + Human recombinant PDK2 (aa178-404) protein

    Predicted band size: 46 kDa

References

ab201964 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (quadriceps muscle)
Total protein in input
100 µg
Immuno-precipitation step
Protein G
Specification
quadriceps muscle

Abcam user community

Verified customer

Submitted Jan 05 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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