Overview

  • Product name

    Anti-PDK2 antibody [EPR1948Y]
    See all PDK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR1948Y] to PDK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human PDK2 aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human heart tissue lysate, mouse heart tissue lysate, rat tissue lysate and human muscle tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab68164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP 1/30.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity

    Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
  • Sequence similarities

    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody
    • mitochondrial antibody
    • PDHK2 antibody
    • PDK2 antibody
    • PDK2_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoform 2 antibody
    • Pyruvate dehydrogenase kinase, isozyme 2 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: PDK2 knockout HAP1 cell lysate (40 µg)
    Lane 3: SH-SY5Y cell lysate (40 ug)
    Lanes 1 - 3: Merged signal (red and green). Green - ab68164 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab68164 was shown to specifically react with PDK2 in wild-type HAP1 cells. No band was observed when PDK2 knockout samples were examined. Wild-type and PDK2 knockout samples were subjected to SDS-PAGE. Ab68164 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) ab216773 and IRDye® 680 Goat anti-Mouse IgG (H + L) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/2000 dilution

    Lane 1 : human heart tissue lysate
    Lane 2 : mouse heart tissue lysate
    Lane 3 : rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    Suggested secondary antibody - goat anti-rabbit HRP (ab6721)

  • ab68164 at 1/100-1/250 dilution staining PDK2 in human muscle tissue sections.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • He Z  et al. MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer. Cell Death Dis 9:505 (2018). Read more (PubMed: 29725130) »
  • Huang S  et al. WAVE3 promotes proliferation, migration and invasion via the AKT pathway in pancreatic cancer. Int J Oncol 53:672-684 (2018). Read more (PubMed: 29845225) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (head and neck cancer)
Permeabilization
Yes - triton x 100
Specification
head and neck cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 06 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10)
Sample
Human Purified protein (head and neck cancer (AMC HN9))
Specification
head and neck cancer (AMC HN9)
Treatment
0 5 15um DCA for 24h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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