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Rabbit polyclonal to PDK3
HumanPredicted to work with:
Mouse, Rat, Chicken, Cow
Recombinant fragment, corresponding to a region within amino acids 7-290 of Human PDK3 (Uniprot ID: Q15120).
- HepG2 whole cell lysate; HepG2 cells; Human Ca922 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 47 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/100 - 1/1000.
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Found almost exclusively in heart and skeletal muscle.
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Information by UniProt
- [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody
- CMTX6 antibody
Western blot - Anti-PDK3 antibody (ab154549)
Anti-PDK3 antibody (ab154549) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK3 antibody (ab154549)
Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue labeling PDK3 with ab154549 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PDK3 antibody (ab154549)
Immunofluorescent analysis of methanol-fixed HepG2 cells labeling PDK3 with ab154549 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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