Key features and details
- Rabbit monoclonal [EP12309] to PDK3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-PDK3 antibody [EP12309]
See all PDK3 primary antibodies
DescriptionRabbit monoclonal [EP12309] to PDK3
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide within Human PDK3 aa 250-350. The exact sequence is proprietary.
Database link: Q15120
- Mouse heart and kidney tissue. Rat Heart tissue and C6 lysates. Raw264.7 cells and cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Tissue specificityFound almost exclusively in heart and skeletal muscle.
Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody
- CMTX6 antibody
Flow cytometric analysis of Raw264.7 cells (paraformaldehyde-fixed, 2%) labeling PDK3 with ab182574 at 1/400 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.
All lanes : Anti-PDK3 antibody [EP12309] (ab182574) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat heart lysate
Lane 4 : C6 lysate
Lane 5 : Raw264.7 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
ab182574 has been referenced in 1 publication.
- Naia L et al. Histone Deacetylase Inhibitors Protect Against Pyruvate Dehydrogenase Dysfunction in Huntington's Disease. J Neurosci 37:2776-2794 (2017). PubMed: 28123081