Overview

  • Product name
    Anti-PDK3 antibody [EP12309]
    See all PDK3 primary antibodies
  • Description
    Rabbit monoclonal [EP12309] to PDK3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human PDK3 aa 250-350. The exact sequence is proprietary.
    Database link: Q15120

  • Positive control
    • Mouse heart and kidney tissue. Rat Heart tissue and C6 lysates. Raw264.7 cells and cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab182574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).
Flow Cyt 1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity
    Found almost exclusively in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody
    • CMTX6 antibody
    • GS1-358P8.4 antibody
    • mitochondrial antibody
    • PDK3 antibody
    • PDK3_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoenzyme 3 antibody
    • Pyruvate dehydrogenase kinase isoform 3 antibody
    • Pyruvate dehydrogenase kinase isozyme 3 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial antibody
    see all

Images

  • Flow cytometric analysis of Raw26.7 cells (paraformaldehyde-fixed, 2%) labeling PDK3 with ab182574 at 1/400 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.

  • All lanes : Anti-PDK3 antibody [EP12309] (ab182574) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat heart lysate
    Lane 4 : C6 lysate
    Lane 5 : Raw264.7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa

References

ab182574 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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