Overview

  • Product name

  • Description

    Rabbit polyclonal to PDK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the C terminal region of Human PDK4

  • Positive control

    • WB: Human skeletal muscle lysate. IHC: human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab89295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/250.

Target

  • Function

    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity

    Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
    • FLJ40832 antibody
    • mitochondrial antibody
    • Pdk4 antibody
    • PDK4_HUMAN antibody
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial antibody
    • Pyruvate dehydrogenase kinase 4 antibody
    • Pyruvate dehydrogenase kinase isoenzyme 4 antibody
    • Pyruvate dehydrogenase kinase isoform 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial antibody
    see all

Images

  • Anti-PDK4 antibody (ab89295) at 1/500 dilution + Human fetal skeletal muscle lysate

    Predicted band size: 46 kDa

  • Anti-PDK4 antibody (ab89295) at 1/500 dilution + Human A549 (lung carcinoma) cell line.

    Predicted band size: 46 kDa

  • Ab89295 staining PDK4 antibody in human gastrointestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • Left panel: ab89295 at 1/100 dilution showing cytoplasmic staining of PDK4 in formalin-fixed, paraffin-embedded human fetal skeletal tissue by Immunohistochemistry.
    Right panel: same, after blocking with the immunizing peptide.

References

This product has been referenced in:

  • Strowitzki MJ  et al. High hepatic expression of PDK4 improves survival upon multimodal treatment of colorectal liver metastases. Br J Cancer 120:675-688 (2019). Read more (PubMed: 30808993) »
  • Yang R  et al. HIF-1a/PDK4/autophagy pathway protects against advanced glycation end-products induced vascular smooth muscle cell calcification. Biochem Biophys Res Commun 517:470-476 (2019). Read more (PubMed: 31376939) »
See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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