Overview

  • Product name
    Anti-PDK4 antibody - N-terminal
    See all PDK4 primary antibodies
  • Description
    Rabbit polyclonal to PDK4 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Mouse PDK4 aa 81-110 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O70571

  • Positive control
    • Mouse lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab172920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 47 kDa.

Target

  • Function
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity
    Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
    • FLJ40832 antibody
    • mitochondrial antibody
    • Pdk4 antibody
    • PDK4_HUMAN antibody
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial antibody
    • Pyruvate dehydrogenase kinase 4 antibody
    • Pyruvate dehydrogenase kinase isoenzyme 4 antibody
    • Pyruvate dehydrogenase kinase isoform 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial antibody
    see all

Images

  • Anti-PDK4 antibody - N-terminal (ab172920) at 1/100 dilution + Mouse lung tissue lysate at 35 µg

    Predicted band size: 47 kDa

References

ab172920 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Dog Cell lysate - whole cell (Skin Fibroblasts)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
40 µg
Specification
Skin Fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 12 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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