Overview

  • Product name
  • Description
    Goat polyclonal to PDLIM1
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    TPPEGYEVVTVFPK

    , corresponding to amino acids 316-329 of Human PDLIM1.

  • Positive control
    • Human lung and heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab17022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa).

Target

  • Function
    Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
  • Tissue specificity
    Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • C terminal LIM domain protein 1 antibody
    • C-terminal LIM domain protein 1 antibody
    • Carboxy terminal LIM domain protein 1 antibody
    • CLIM 1 antibody
    • CLIM1 antibody
    • CLP 36 antibody
    • CLP36 antibody
    • Elfin antibody
    • Enigma antibody
    • Enigma homolog antibody
    • hCLIM 1 antibody
    • hCLIM1 antibody
    • LIM domain protein CLP 36 antibody
    • LIM domain protein CLP-36 antibody
    • LIM domain protein CLP36 antibody
    • PDLI1_HUMAN antibody
    • PDLIM 1 antibody
    • Pdlim1 antibody
    • PDZ and LIM domain 1 antibody
    • PDZ and LIM domain protein 1 antibody
    see all

Images

  • Anti-PDLIM1 antibody (ab17022) at 0.1 µg/ml + Human lung lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    ab17022 staining (0.1ug/ml) on human lung lysate (35ug). Primary incubated for 1 hour.

References

This product has been referenced in:
  • Naegle KM  et al. Robust co-regulation of tyrosine phosphorylation sites on proteins reveals novel protein interactions. Mol Biosyst 8:2771-82 (2012). Read more (PubMed: 22851037) »
  • Gupta S  et al. CLP36 is a negative regulator of glycoprotein VI signaling in platelets. Circ Res 111:1410-20 (2012). Read more (PubMed: 22955732) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Myotubes)
Loading amount
40 µg
Specification
Mouse Myotubes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Ilenia Bertipaglia

Verified customer

Submitted Jun 19 2006

Answer

Thank you for your email and I'm sorry to hear that you are experiencing some difficulty with these antibodies. All 3 of these antibodies have been successfully tested for application in Western blotting. However, these antibodies have not yet been tested for application in IHC to our knowledge. They very well may not be suitable for this particular application. I do suggest that you submit Abreviews regarding your experiences with these antibodies in IHC as your feedback is extremely useful for us as well as other customers. To submit an Abreview, just click on the Abreviews tab located on the online product datasheet and then click on the submit an Abreview link. In exchange for an Abreview we will award 50 Abpoints (an additional 100 Abpoints if an image is submitted) which can be exchanged for a variety of awards. In order to check to see if your secondary antibody is causing the background that you are seeing, you can test this by omitting primary antibody in your protocol and testing with secondary antibody only. If background appears, change either the secondary antibody or the blocking agent. At the bottom of the online datasheets for these antibodies are a list of compatible secondaries (such as ab6741) which are suitable for use with these primary antibodies. For ab1301 (Goat polyclonal to LASP1), an approximate 30 kDa band was seen in Mouse Brain extracts. I would therefore suggest using this as a positive control. I hope this helps. Please don't hesitate to contact us again if you need additional assistance with these antibodies. Happy New Year!

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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