Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab17022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa).|
FunctionCytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Tissue specificityStrongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
- Information by UniProt
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
Anti-PDLIM1 antibody (ab17022) at 0.1 µg/ml + Human lung lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab17022 staining (0.1ug/ml) on human lung lysate (35ug). Primary incubated for 1 hour.
This product has been referenced in:
- Naegle KM et al. Robust co-regulation of tyrosine phosphorylation sites on proteins reveals novel protein interactions. Mol Biosyst 8:2771-82 (2012). Read more (PubMed: 22851037) »
- Gupta S et al. CLP36 is a negative regulator of glycoprotein VI signaling in platelets. Circ Res 111:1410-20 (2012). Read more (PubMed: 22955732) »