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Rabbit monoclonal [EPR7187] to PDLIM1
ICC/IF more details Unsuitable for:
IHC-P or IP
Mouse, Rat, Human
Synthetic peptide within Human PDLIM1 aa 200-300. The exact sequence is proprietary.
Database link: O00151
Jurkat cell line lysate
HeLa cell line lysate and cells
293T cell line lysate
Caco-2 cell line lysate
Saos-2 cell line lysate
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/250 - 1/500.
Is unsuitable for IHC-P or IP.
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
Information by UniProt
C terminal LIM domain protein 1 antibody
C-terminal LIM domain protein 1 antibody
Carboxy terminal LIM domain protein 1 antibody
Western blot - Anti-PDLIM1 antibody [EPR7187] (ab126628)
All lanes : Anti-PDLIM1 antibody [EPR7187] (ab126628) at 1/1000 dilution Lane 1 : Jurkat cell line lysate Lane 2 : HeLa cell line lysate Lane 3 : 293T cell line lysate Lane 4 : Caco 2 cell line lysate Lane 5 : Saos 2 cell line lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1 antibody [EPR7187] (ab126628)
ab126628, at 1/250, staining PDLIM1 in HeLa cells by Immunofluorescence.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"