Product nameAnti-PDLIM1/CLP36 antibody [EPR7186(B)]
See all PDLIM1/CLP36 primary antibodies
DescriptionRabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36
Tested applicationsSuitable for: WB, IP, ICC, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PDLIM1/CLP36 aa 50-150. The exact sequence is proprietary.
- Human heart, L6, 293T, Caco 2, Saos 2, C2C12, Rat lung and Mouse lung lysates; Saos 2 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.54 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
|IP||1/10 - 1/100.|
|ICC||1/250 - 1/500.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Tissue specificityStrongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
- Information by UniProt
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
All lanes : Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015) at 1/1000 dilution
Lane 1 : Human heart cell lysate
Lane 2 : L6 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco 2 cell lysate
Lane 5 : Saos 2 cell lysate
Lane 6 : C2C12 cell lysate
Lane 7 : Rat lung cell lysate
Lane 8 : Mouse lung cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 36 kDa
ab129015, at 1/100 dilution, staining PDLIM1/CLP36 in permeabilized Saos 2 cells by Flow Cytometry or a Rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
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