Overview

  • Product name

    Anti-PDLIM1/CLP36 antibody [EPR7186(B)]
    See all PDLIM1/CLP36 primary antibodies
  • Description

    Rabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PDLIM1/CLP36 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human heart, L6, 293T, Caco 2, Saos 2, C2C12, Rat lung and Mouse lung lysates; Saos 2 cells.
  • General notes

     

     

     This product was previously labelled as PDLIM1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP 1/10 - 1/100.
ICC 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
    • Tissue specificity

      Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
    • Sequence similarities

      Contains 1 LIM zinc-binding domain.
      Contains 1 PDZ (DHR) domain.
    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
    • Information by UniProt
    • Database links

    • Alternative names

      • C terminal LIM domain protein 1 antibody
      • C-terminal LIM domain protein 1 antibody
      • Carboxy terminal LIM domain protein 1 antibody
      • CLIM 1 antibody
      • CLIM1 antibody
      • CLP 36 antibody
      • CLP36 antibody
      • Elfin antibody
      • Enigma antibody
      • Enigma homolog antibody
      • hCLIM 1 antibody
      • hCLIM1 antibody
      • LIM domain protein CLP 36 antibody
      • LIM domain protein CLP-36 antibody
      • LIM domain protein CLP36 antibody
      • PDLI1_HUMAN antibody
      • PDLIM 1 antibody
      • Pdlim1 antibody
      • PDZ and LIM domain 1 antibody
      • PDZ and LIM domain protein 1 antibody
      see all

    Images

    • All lanes : Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015) at 1/1000 dilution

      Lane 1 : Human heart cell lysate
      Lane 2 : L6 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Caco 2 cell lysate
      Lane 5 : Saos 2 cell lysate
      Lane 6 : C2C12 cell lysate
      Lane 7 : Rat lung cell lysate
      Lane 8 : Mouse lung cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 36 kDa

    • ab129015, at 1/100 dilution, staining PDLIM1/CLP36 in permeabilized Saos 2 cells by Flow Cytometry or a Rabbit IgG negative control (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Ono R  et al. PDLIM1 inhibits NF-?B-mediated inflammatory signaling by sequestering the p65 subunit of NF-?B in the cytoplasm. Sci Rep 5:18327 (2015). Read more (PubMed: 26679095) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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