Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36
- Suitable for: WB, IP, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-PDLIM1/CLP36 antibody [EPR7186(B)]
See all PDLIM1/CLP36 primary antibodies
DescriptionRabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PDLIM1/CLP36 aa 50-150. The exact sequence is proprietary.
- Human heart, L6, 293T, Caco 2, Saos 2, C2C12, Rat lung and Mouse lung lysates; Saos 2 cells. ICC/IF: 293T cells
This product was previously labelled as PDLIM1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.54 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab129015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Tissue specificityStrongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
- Information by UniProt
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
All lanes : Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015) at 1/1000 dilution
Lane 1 : Human heart cell lysate
Lane 2 : L6 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco 2 cell lysate
Lane 5 : Saos 2 cell lysate
Lane 6 : C2C12 cell lysate
Lane 7 : Rat lung cell lysate
Lane 8 : Mouse lung cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence analysis of 293T (human embryonic kidney epithelial cell) cells labeling PDLIM1/CLP36 with purified ab129015 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
ab129015, at 1/100 dilution, staining PDLIM1/CLP36 in permeabilized Saos 2 cells by Flow Cytometry or a Rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129015 has been referenced in 1 publication.
- Ono R et al. PDLIM1 inhibits NF-?B-mediated inflammatory signaling by sequestering the p65 subunit of NF-?B in the cytoplasm. Sci Rep 5:18327 (2015). PubMed: 26679095