Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-PDLIM1/CLP36 antibody [EPR7186(B)] - BSA and Azide free
See all PDLIM1/CLP36 primary antibodies
DescriptionRabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PDLIM1/CLP36 aa 50-150. The exact sequence is proprietary.
- ICC/IF: 293T cells
ab240039 is the carrier-free version of ab129015 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240039 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PDLIM1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 9.54 x 10 -11 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab240039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
FunctionCytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Tissue specificityStrongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
- Information by UniProt
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
Immunocytochemistry/ Immunofluorescence analysis of 293T (human embryonic kidney epithelial cell) cells labeling PDLIM1/CLP36 with purified ab129015 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129015).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240039 has not yet been referenced specifically in any publications.