RecombinantRabMAb

Recombinant Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

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Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
  • Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7187] to PDLIM1/CLP36
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-PDLIM1/CLP36 antibody [EPR7187]
    See all PDLIM1/CLP36 primary antibodies

  • Description

    Rabbit monoclonal [EPR7187] to PDLIM1/CLP36

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PDLIM1/CLP36 aa 200-300. The exact sequence is proprietary.
    Database link: O00151

  • Positive control

    • Jurkat cell line lysate HeLa cell line lysate and cells 293T cell line lysate Caco-2 cell line lysate Saos-2 cell line lysate

  • General notes

     

     

     This product was previously labelled as PDLIM1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.

    • Tissue specificity

      Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

    • Sequence similarities

      Contains 1 LIM zinc-binding domain.
      Contains 1 PDZ (DHR) domain.

    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • C terminal LIM domain protein 1 antibody
      • C-terminal LIM domain protein 1 antibody
      • Carboxy terminal LIM domain protein 1 antibody
      • CLIM 1 antibody
      • CLIM1 antibody
      • CLP 36 antibody
      • CLP36 antibody
      • Elfin antibody
      • Enigma antibody
      • Enigma homolog antibody
      • hCLIM 1 antibody
      • hCLIM1 antibody
      • LIM domain protein CLP 36 antibody
      • LIM domain protein CLP-36 antibody
      • LIM domain protein CLP36 antibody
      • PDLI1_HUMAN antibody
      • PDLIM 1 antibody
      • Pdlim1 antibody
      • PDZ and LIM domain 1 antibody
      • PDZ and LIM domain protein 1 antibody
      see all

    Images

    • Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
      Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
      All lanes : Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628) at 1/1000 dilution

      Lane 1 : Jurkat cell line lysate
      Lane 2 : HeLa cell line lysate
      Lane 3 : 293T cell line lysate
      Lane 4 : Caco 2 cell line lysate
      Lane 5 : Saos 2 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
      Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

      ab126628, at 1/250, staining PDLIM1/CLP36 in HeLa cells by Immunofluorescence.

    • Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
      Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

    References (1)

    Publishing research using ab126628? Please let us know so that we can cite the reference in this datasheet.

    ab126628 has been referenced in 1 publication.

    • Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . PubMed: 27078885

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