Recombinant Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7187] to PDLIM1/CLP36
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-PDLIM1/CLP36 antibody [EPR7187]
See all PDLIM1/CLP36 primary antibodies -
Description
Rabbit monoclonal [EPR7187] to PDLIM1/CLP36 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide within Human PDLIM1/CLP36 aa 200-300. The exact sequence is proprietary.
Database link: O00151 -
Positive control
- Jurkat cell line lysate HeLa cell line lysate and cells 293T cell line lysate Caco-2 cell line lysate Saos-2 cell line lysate
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General notes
This product was previously labelled as PDLIM1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7187 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
WB |
Human
|
All applications |
Mouse
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
|
|
ICC/IF |
1/250 - 1/500.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. -
Tissue specificity
Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers. - Information by UniProt
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Database links
- Entrez Gene: 9124 Human
- Entrez Gene: 54132 Mouse
- Entrez Gene: 54133 Rat
- Omim: 605900 Human
- SwissProt: O00151 Human
- SwissProt: O70400 Mouse
- SwissProt: Q3TZ17 Mouse
- SwissProt: P52944 Rat
see all -
Alternative names
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
see all
Images
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All lanes : Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628) at 1/1000 dilution
Lane 1 : Jurkat cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : 293T cell line lysate
Lane 4 : Caco 2 cell line lysate
Lane 5 : Saos 2 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 36 kDa -
ab126628, at 1/250, staining PDLIM1/CLP36 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
References (1)
ab126628 has been referenced in 1 publication.
- Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . PubMed: 27078885