Name: Anti-PDLIM1/CLP36 antibody [EPR7187]
Brand: RabMAb
SKU: ab126628

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Product name

Anti-PDLIM1/CLP36 antibody [EPR7187]
See all PDLIM1/CLP36 primary antibodies
Description

Rabbit monoclonal [EPR7187] to PDLIM1/CLP36
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human PDLIM1/CLP36 aa 200-300. The exact sequence is proprietary.
Database link: O00151
Positive control
- Jurkat cell line lysate HeLa cell line lysate and cells 293T cell line lysate Caco-2 cell line lysate Saos-2 cell line lysate
General notes

This product was previously labelled as PDLIM1

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7187
Isotype

IgG
Research areas

Alternative Versions
- Anti-PDLIM1/CLP36 antibody [EPR7187] - BSA and Azide free (ab248135)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
Recombinant Protein
- Recombinant Human PDLIM1/CLP36 protein (ab177676)

Our Abpromise guarantee covers the use of ab126628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

1/1000 - 1/10000. Predicted molecular weight: 36 kDa.

Flow Cyt

1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF

1/250 - 1/500.

Application notes

Is unsuitable for IHC-P or IP.

Function

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Tissue specificity

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
Sequence similarities

Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cellular localization

Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
Target information above from: UniProt accession O00151 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9124 Human
- Entrez Gene: 54132 Mouse
- Entrez Gene: 54133 Rat
- Omim: 605900 Human
- SwissProt: O00151 Human
- SwissProt: O70400 Mouse
- SwissProt: Q3TZ17 Mouse
- SwissProt: P52944 Rat
- Unigene: 368525 Human
- Unigene: 5567 Mouse
- Unigene: 11170 Rat
see all
Alternative names
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
- CLIM 1 antibody
- CLIM1 antibody
- CLP 36 antibody
- CLP36 antibody
- Elfin antibody
- Enigma antibody
- Enigma homolog antibody
- hCLIM 1 antibody
- hCLIM1 antibody
- LIM domain protein CLP 36 antibody
- LIM domain protein CLP-36 antibody
- LIM domain protein CLP36 antibody
- PDLI1_HUMAN antibody
- PDLIM 1 antibody
- Pdlim1 antibody
- PDZ and LIM domain 1 antibody
- PDZ and LIM domain protein 1 antibody
see all

Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

All lanes : Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628) at 1/1000 dilution

Lane 1 : Jurkat cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : 293T cell line lysate
Lane 4 : Caco 2 cell line lysate
Lane 5 : Saos 2 cell line lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

ab126628, at 1/250, staining PDLIM1/CLP36 in HeLa cells by Immunofluorescence.

Flow cytometry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . Read more (PubMed: 27078885) »

See 1 Publication for this product

Publishing research using ab126628? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As