Recombinant
RabMAb

Recombinant Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

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Overview

  • Product name

    Anti-PDLIM1/CLP36 antibody [EPR7187]
    See all PDLIM1/CLP36 primary antibodies

  • Description

    Rabbit monoclonal [EPR7187] to PDLIM1/CLP36

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PDLIM1/CLP36 aa 200-300. The exact sequence is proprietary.
    Database link: O00151

  • Positive control

    • Jurkat cell line lysate HeLa cell line lysate and cells 293T cell line lysate Caco-2 cell line lysate Saos-2 cell line lysate

  • General notes

     

     

     This product was previously labelled as PDLIM1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab126628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.

    • Tissue specificity

      Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

    • Sequence similarities

      Contains 1 LIM zinc-binding domain.
      Contains 1 PDZ (DHR) domain.

    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • C terminal LIM domain protein 1 antibody
      • C-terminal LIM domain protein 1 antibody
      • Carboxy terminal LIM domain protein 1 antibody
      • CLIM 1 antibody
      • CLIM1 antibody
      • CLP 36 antibody
      • CLP36 antibody
      • Elfin antibody
      • Enigma antibody
      • Enigma homolog antibody
      • hCLIM 1 antibody
      • hCLIM1 antibody
      • LIM domain protein CLP 36 antibody
      • LIM domain protein CLP-36 antibody
      • LIM domain protein CLP36 antibody
      • PDLI1_HUMAN antibody
      • PDLIM 1 antibody
      • Pdlim1 antibody
      • PDZ and LIM domain 1 antibody
      • PDZ and LIM domain protein 1 antibody
      see all

    Images

    • Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
      Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
      All lanes : Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628) at 1/1000 dilution

      Lane 1 : Jurkat cell line lysate
      Lane 2 : HeLa cell line lysate
      Lane 3 : 293T cell line lysate
      Lane 4 : Caco 2 cell line lysate
      Lane 5 : Saos 2 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
      Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)

      ab126628, at 1/250, staining PDLIM1/CLP36 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    • Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . Read more (PubMed: 27078885) »
    See 1 Publication for this product

    Publishing research using ab126628? Please let us know so that we can cite the reference in this datasheet.

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