Anti-PDLIM5/ENH antibody (ab85967)
Key features and details
- Rabbit polyclonal to PDLIM5/ENH
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDLIM5/ENH antibody
See all PDLIM5/ENH primary antibodies -
Description
Rabbit polyclonal to PDLIM5/ENH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human PDLIM5/ENH aa 500-600 (C terminal).
Database link: Q96HC4 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 64 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses. -
Tissue specificity
Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression. -
Sequence similarities
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites. - Information by UniProt
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Database links
- Entrez Gene: 503621 Cow
- Entrez Gene: 10611 Human
- Entrez Gene: 56376 Mouse
- Entrez Gene: 64353 Rat
- Omim: 605904 Human
- SwissProt: Q96HC4 Human
- SwissProt: Q8CI51 Mouse
- SwissProt: Q62920 Rat
see all -
Alternative names
- ENH antibody
- ENH1 antibody
- Enigma homolog antibody
see all
Images
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All lanes : Anti-PDLIM5/ENH antibody (ab85967) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 64 kDa
Exposure time: 30 seconds -
1mg HeLa whole cell lysate was immunoprecipitated using ab85967 at 3µg. 20% of IP was loaded/lane and PDLIM5/ENH was detected with ab85967 at 1µg/ml (lane 1) or a control IgG (lane 2).
Detection: chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85967 has not yet been referenced specifically in any publications.