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Rabbit polyclonal to PDLIM5
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide dessigned within residues: EAGDM FLEA LGYTWH DTC FVCSVCC ES LEGQTFFS K KDKPLCKKH AHSVNF, corresponding to C terminal amino acids 546-596 of Human PDLIM5 (NP_006448.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
Use at 2-5 µg/mg of lysate.
May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses.
Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites.
Information by UniProt
Enigma homolog antibody
Western blot - Anti-PDLIM5 antibody (ab85967)
All lanes : Anti-PDLIM5 antibody (ab85967) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Predicted band size: 64 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-PDLIM5 antibody (ab85967)
1mg HeLa whole cell lysate was immunoprecipitated using ab85967 at 3µg. 20% of IP was loaded/lane and PDLIM5 was detected with ab85967 at 1µg/ml (lane 1) or a control IgG (lane 2).
Detection: chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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