Product nameAnti-PDLIM5/ENH antibody
See all PDLIM5/ENH primary antibodies
DescriptionRabbit polyclonal to PDLIM5/ENH
This antibody detects endogenous level of total PDLIM5/ENH protein.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment corresponding to Human PDLIM5/ENH (C terminal).
Database link: Q96HC4
- HL60, BT474, HepG2, MCF7 cell extracts. Mouse lung, heart, kidney and uterus tissue extracts. U20S cells.
This product was previously labelled as PDLIM5
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were purified by affinity purification using immunogen.
- HepG2 whole cell lysate (ab166833)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- MCF7 whole cell lysate (ab29537)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- HepG2 whole cell lysate (ab7900)
- HL60 whole cell lysate (ab7914)
Our Abpromise guarantee covers the use of ab196559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 64 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionMay play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses.
Tissue specificityHeart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
Sequence similaritiesContains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Cellular localizationCell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites.
- Information by UniProt
- ENH antibody
- ENH1 antibody
- Enigma homolog antibody
All lanes : Recombinant Human Superoxide Dismutase 1 protein (ab159559) at 1/500 dilution
Lane 1 : HL60 extracts
Lane 2 : BT474 extracts
Lane 3 : HepG2 extracts
Lane 4 : MCF7 extracts
Lane 5 : Mouse lung extracts
Lane 6 : Mouse heart extracts
Lane 7 : Mouse kidney extracts
Lane 8 : Mouse uterus extracts
Predicted band size: 64 kDa
Immunofluorescence analysis of U20S cells labeling PDLIM5/ENH using ab196559, at 1/50 dilution. Lower panel shows DAPI for nuclear staining (Blue).
ab196559 has not yet been referenced specifically in any publications.