Key features and details
- Rabbit polyclonal to PDLIM5/ENH
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDLIM5/ENH antibody
See all PDLIM5/ENH primary antibodies
DescriptionRabbit polyclonal to PDLIM5/ENH
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PDLIM5/ENH aa 375-425. The exact sequence is proprietary.
Database link: Q96HC4
- IP: HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Use at 2-5 µg/mg of lysate.
FunctionMay play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses.
Tissue specificityHeart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
Sequence similaritiesContains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Cellular localizationCell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites.
- Information by UniProt
- ENH antibody
- ENH1 antibody
- Enigma homolog antibody
PDLIM5/ENH was immunoprecipitated from 1 mg Hela whole cell lysate using ab264223 at 3 µg per reaction. Western blot was performed on the immunioprecipitate using an alternative anti PDLIM5/ENH antibody at 1 µg/ml.
Lane 1: PDLIM5/ENH IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264223 has not yet been referenced specifically in any publications.