Overview

  • Product name

  • Description

    Rabbit polyclonal to PDLIM5/ENH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human PDLIM5/ENH aa 546-596 (C terminal).
    Sequence:

    EAGDM FLEALGYTWH DTCFVCSVCC ESLEGQTFFS KKDKPLCKKH AHSVNF


    Database link: NP_006448.2

  • Positive control

    • HeLa whole cell lysate
  • General notes

     This product was previously labelled as PDLIM5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab85967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses.
  • Tissue specificity

    Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
  • Sequence similarities

    Contains 3 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites.
  • Information by UniProt
  • Database links

  • Alternative names

    • ENH antibody
    • ENH1 antibody
    • Enigma homolog antibody
    • Enigma like LIM domain protein antibody
    • Enigma like PDZ and LIM domains protein antibody
    • Enigma-like PDZ and LIM domains protein antibody
    • L 9 antibody
    • L9 antibody
    • LIM antibody
    • PDLI5_HUMAN antibody
    • PDLIM5 antibody
    • PDZ and LIM domain 5 antibody
    • PDZ and LIM domain protein 5 antibody
    see all

Images

  • All lanes : Anti-PDLIM5/ENH antibody (ab85967) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 64 kDa


    Exposure time: 30 seconds
  • 1mg HeLa whole cell lysate was immunoprecipitated using ab85967 at 3µg. 20% of IP was loaded/lane and PDLIM5/ENH  was detected with ab85967 at 1µg/ml (lane 1) or a control IgG (lane 2).
    Detection: chemiluminescence with exposure time of 10 seconds.

References

ab85967 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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