Key features and details
- Rabbit polyclonal to PDLIM5/ENH
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDLIM5/ENH antibody
See all PDLIM5/ENH primary antibodies
DescriptionRabbit polyclonal to PDLIM5/ENH
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
- HeLa whole cell lysate
This product was previously labelled as PDLIM5
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 64 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses.
Tissue specificityHeart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
Sequence similaritiesContains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Cellular localizationCell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites.
- Information by UniProt
- ENH antibody
- ENH1 antibody
- Enigma homolog antibody
All lanes : Anti-PDLIM5/ENH antibody (ab85967) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 64 kDa
Exposure time: 30 seconds
1mg HeLa whole cell lysate was immunoprecipitated using ab85967 at 3µg. 20% of IP was loaded/lane and PDLIM5/ENH was detected with ab85967 at 1µg/ml (lane 1) or a control IgG (lane 2).
Detection: chemiluminescence with exposure time of 10 seconds.
ab85967 has not yet been referenced specifically in any publications.