Key features and details
- Rabbit polyclonal to PDLIM7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PDLIM7 antibody
See all PDLIM7 primary antibodies
DescriptionRabbit polyclonal to PDLIM7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Bat
Synthetic peptide corresponding to a region between amino acids 125 and 175 of Human PDLIM7 (NP_005442.2)
- Hela cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway.
Tissue specificityIsoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver.
Sequence similaritiesContains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
DomainThe LIM zinc-binding 2 (LIM 2) domain interacts with TBX4.
The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5.
Anchored to cell periphery via its N-terminal PDZ domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments.
- Information by UniProt
- 1110003B01Rik antibody
- Enigma antibody
- LIM domain protein antibody
All lanes : Anti-PDLIM7 antibody (ab86065) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 30 seconds
Detection of PDLIM7 by Western blot of Immunprecipitate.
ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab86065 has been referenced in 1 publication.
- Ajeian JN et al. Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl 10:51-7 (2016). PubMed: 26147903