Overview

  • Product name

  • Description

    Rabbit polyclonal to PDP1/PDP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Fusion protein corresponding to Human PDP1/PDP (C terminal). The identity of the protein fusion partner is GST (BC047619).
    Database link: Q9P0J1

  • Positive control

    • Human bladder carcinoma tissue lysate; Human thyroid cancer tissue.
  • General notes

     This product was previously labelled as PDP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab198261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/200 - 1/1000. Predicted molecular weight: 61 kDa.

Target

  • Function

    Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • Involvement in disease

    Defects in PDP1 are the cause of pyruvate dehydrogenase phosphatase deficiency (PDP deficiency) [MIM:608782]. PDP deficiency results in lactic acidosis leading to neurological dysfunction.
  • Sequence similarities

    Belongs to the PP2C family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Pyruvate dehydrogenase [acetyl transferring]] phosphatase 1, mitochondrial antibody
    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1 antibody
    • FLJ32517 antibody
    • Gm1024 antibody
    • MGC119646 antibody
    • mitochondrial antibody
    • PDH antibody
    • PDP 1 antibody
    • PDP antibody
    • Pdp1 antibody
    • PDP1_HUMAN antibody
    • PDPC 1 antibody
    • PDPC antibody
    • PPM2C antibody
    • Protein phosphatase 2C antibody
    • protein phosphatase 2C, magnesium dependent, catalytic subunit antibody
    • Pyruvate dehydrogenase (Lipoamide) phosphatase phosphatase antibody
    • Pyruvate dehydrogenase phosphatase catalytic subunit 1 antibody
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 1 antibody
    • RP23 203A12.1 antibody
    see all

Images

  • Anti-PDP1/PDP antibody (ab198261) at 1/300 dilution + Human bladder carcinoma tissue lysate at 40 µg

    Predicted band size: 61 kDa



    Gel: 6% SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PDP1/PDP with ab198261 at 1/25 dilution.

References

ab198261 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - other (MCF7 mitochondrial lysate and MCF7 whole cell lysa)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
MCF7 mitochondrial lysate and MCF7 whole cell lysa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 07 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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